| High-resolution magnetic resonance colonography and dynamic contrast-enhanced magnetic resonance imaging in a murine model of colitis. | |
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MedLine Citation:
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PMID: 20373393 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inflammatory bowel disease, including ulcerative colitis, is characterized by persistent or recurrent inflammation and can progress to colon cancer. Colitis is difficult to detect and monitor noninvasively. The goal of this work was to develop a preclinical imaging method for evaluating colitis. Herein, we report improved MRI methods for detecting and characterizing colitis noninvasively in mice, using high-resolution in vivo MR images and dynamic contrast-enhanced MRI studies, which were confirmed by histologic studies in a murine model of colitis. C57Bl6/J male mice were treated with 2.5% dextran sulfate sodium in their drinking water for 5 days to induce colitis. MR images were acquired using a 9.4-T Bruker scanner from 5-25 days following dextran sulfate sodium treatment. In dynamic contrast-enhanced MRI studies, Gd uptake (K(trans)) and its distribution (v(e)) were measured in muscle and normal and inflamed colons after administering Gd-diethyltriaminepentaacetic acid (Gd-DTPA). T(2)-weighted MR images distinguished normal colon from diffusely thickened colonic wall occurring in colitis (P <0.0005) and correlated with histologic features. Values of K(trans) and v(e) obtained from dynamic contrast-enhanced MRI were also significantly different in inflamed colons compared to normal colon (P < 0.0005). The results demonstrate that both T(2)-weighted anatomic imaging and quantitative analysis of dynamic contrast-enhanced MRI data can successfully distinguish colitis from normal colon in mice. |
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Authors:
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Devkumar Mustafi; Xiaobing Fan; Urszula Dougherty; Marc Bissonnette; Gregory S Karczmar; Aytekin Oto; John Hart; Erica Markiewicz; Marta Zamora |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 63 ISSN: 1522-2594 ISO Abbreviation: Magn Reson Med Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-07 Completed Date: 2010-07-15 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 922-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA. dmustafi@uchicago.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Area Under Curve Colitis / diagnosis* Contrast Media Disease Models, Animal Gadolinium DTPA Image Processing, Computer-Assisted Immunohistochemistry Magnetic Resonance Imaging / methods* Male Mice Mice, Inbred C57BL |
| Grant Support | |
ID/Acronym/Agency:
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2R01 CA36745/CA/NCI NIH HHS; P30 DK042086-19S1/DK/NIDDK NIH HHS; P30 DK42086/DK/NIDDK NIH HHS; R01 CA036745-24/CA/NCI NIH HHS; R33 CA100996/CA/NCI NIH HHS; R33 CA100996-04/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 122795-43-1/gadodiamide; 80529-93-7/Gadolinium DTPA |
| Comments/Corrections | |
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