Document Detail


High-resolution magnetic resonance angiography in the mouse using a nanoparticle blood-pool contrast agent.
MedLine Citation:
PMID:  19902507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-resolution magnetic resonance angiography is already a useful tool for studying mouse models of human disease. Magnetic resonance angiography in the mouse is typically performed using time-of-flight contrast. In this work, a new long-circulating blood-pool contrast agent-a liposomal nanoparticle with surface-conjugated gadolinium (SC-Gd liposomes)-was evaluated for use in mouse neurovascular magnetic resonance angiography. A total of 12 mice were imaged. Scan parameters were optimized for both time-of-flight and SC-Gd contrast. Compared to time-of-flight contrast, SC-Gd liposomes (0.08 mmol/kg) enabled improved small-vessel contrast-to-noise ratio, larger field of view, shorter scan time, and imaging of venous structures. For a limited field of view, time-of-flight and SC-Gd were not significantly different; however, SC-Gd provided better contrast-to-noise ratio when the field of view encompassed the whole brain (P < 0.001) or the whole neurovascular axis (P < 0.001). SC-Gd allowed acquisition of high-resolution magnetic resonance angiography (52 x 52 x 100 micrometer(3) or 0.27 nL), with 123% higher (P < 0.001) contrast-to-noise ratio in comparable scan time ( approximately 45 min). Alternatively, SC-Gd liposomes could be used to acquire high-resolution magnetic resonance angiography (0.27 nL) with 32% higher contrast-to-noise ratio (P < 0.001) in 75% shorter scan time (12 min).
Authors:
Gabriel P Howles; Ketan B Ghaghada; Yi Qi; Srinivasan Mukundan; G Allan Johnson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  62     ISSN:  1522-2594     ISO Abbreviation:  Magn Reson Med     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-30     Completed Date:  2010-02-22     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1447-56     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
Affiliation:
Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebral Arteries / anatomy & histology*
Contrast Media / chemistry*
Gadolinium / chemistry,  diagnostic use*
Image Enhancement / methods*
Liposomes / chemistry
Magnetic Resonance Angiography / methods*
Male
Mice
Mice, Inbred C57BL
Nanoparticles / chemistry*,  diagnostic use*
Grant Support
ID/Acronym/Agency:
P41 RR005959/RR/NCRR NIH HHS; P41 RR005959-19/RR/NCRR NIH HHS; U24 CA092656/CA/NCI NIH HHS; U24 CA092656-09/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Liposomes; 7440-54-2/Gadolinium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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