Document Detail

High-resolution integrated map encompassing the breast cancer loss of heterozygosity region on human chromosome 16q22.1.
MedLine Citation:
PMID:  11161777     Owner:  NLM     Status:  MEDLINE    
Loss of heterozygosity (LOH) on the long arm of human chromosome 16 is a common genetic alteration observed in both invasive ductal and invasive lobular breast carcinomas. We have generated a high-resolution integrated map encompassing the smallest region of LOH overlap within chromosome 16q22.1 (SRO2). Southern hybridization experiments using more than 140 probes resulted in the assembly of 152 bacterial large-insert clones into a 2.8-Mb contig covering SRO2. The structure of the contig was verified by long-range mapping using total human genomic DNA, and the contig orientation was determined by fluorescence in situ hybridization. A total of 68 transcripts have been identified in the map. One of the genes residing within SRO2 is the E-cadherin gene, CDH1, which has previously been shown to be mutated in lobular breast carcinomas, resulting in loss of E-cadherin expression. In most cases of ductal carcinoma, which is the major mammary cancer type, E-cadherin is normally expressed, suggesting that other genes within 16q22.1 are involved in the development of this tumor subtype. The high-resolution map presented in this study provides a valuable resource for identification of tumor suppressor genes expected to be involved in the etiology of breast carcinomas.
E Frengen; P Rocca-Serra; S Shaposhnikov; L Taine; J Thorsen; C Bepoldin; M Krekling; D Lafon; K K Aas; A A El Monéim; H Johansen; M Longy; H Prydz; F Dorion-Bonnet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  70     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-85     Citation Subset:  IM    
The Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AZ081512;  AZ081513;  AZ081514;  AZ081515;  AZ081516;  AZ081517;  AZ081518;  AZ081519;  AZ081520;  AZ081521;  AZ081522;  AZ081523;  AZ081524;  AZ081525;  AZ081526;  AZ081527;  AZ081528;  AZ081529;  AZ081530;  AZ081531;  AZ081532;  AZ081533;  AZ081534;  AZ081535;  AZ081536;  AZ081537;  AZ081538;  AZ081539;  AZ081540;  AZ081541;  AZ081542;  AZ081543;  AZ081544;  AZ081545;  AZ081546;  AZ081547;  AZ081548;  AZ081549;  AZ081550;  AZ081551;  AZ081552;  AZ081553;  AZ081554;  AZ081555;  AZ081556;  AZ081557;  AZ081558;  AZ081559;  AZ081560;  AZ081561;  AZ081562;  AZ081563;  AZ081564;  AZ081565;  AZ081566;  AZ081567;  AZ081568;  AZ081569;  AZ081570;  AZ081571;  AZ081572;  AZ081573;  AZ081574;  AZ081575;  AZ081576;  AZ081577;  AZ081578;  AZ081579;  AZ081580;  AZ081581;  AZ081582;  AZ081583;  AZ081584;  AZ081585;  AZ081586;  AZ081587;  AZ081588;  AZ081589;  AZ081590;  AZ081591;  AZ081592;  AZ081593;  AZ081594;  AZ081595
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MeSH Terms
Breast Neoplasms / genetics*
Chromosomes, Human, Pair 16*
Genes, Tumor Suppressor
Genetic Markers
Loss of Heterozygosity*
Microsatellite Repeats / genetics
Molecular Sequence Data
Physical Chromosome Mapping*
Polymerase Chain Reaction
Restriction Mapping
Reg. No./Substance:
0/Genetic Markers

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