Document Detail


High resolution cell lineage tracing reveals developmental variability in leech.
MedLine Citation:
PMID:  19924812     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Knowing the normal patterns of embryonic cell proliferation, migration, and differentiation is a cornerstone for understanding development. Yet for most species, the precision with which embryonic cell lineages can be determined is limited by technical considerations (the large numbers of cells, extended developmental times, opacity of the embryos), and these are exacerbated by the inherent variability of the lineages themselves. Here, we present an improved method of cell lineage tracing in the leech Helobdella, driving the expression of a nuclearly localized histone H2B:GFP (green fluorescent protein) fusion protein in selected lineages by microinjection of a plasmid vector. This construct generates a long lasting and minimally mosaic signal with single cell resolution, and does not disrupt the development of most lineages tested. We have validated this technique by elucidating details of cell lineages contributing to segmental and prostomial tissues that could not be observed with standard dextran lineage tracers.
Authors:
Stephanie E Gline; Dian-Han Kuo; Alberto Stolfi; David A Weisblat
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  238     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-01     Completed Date:  2010-03-15     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3139-51     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Cell Differentiation / physiology
Cell Division / physiology
Cell Lineage* / genetics,  physiology
Embryo, Nonmammalian
Embryonic Development / genetics,  physiology
Fluorescent Antibody Technique / methods
Gene Expression Regulation, Developmental
Green Fluorescent Proteins / genetics,  metabolism
Individuality
Leeches / embryology*,  genetics,  metabolism
Models, Biological
Organ Specificity / physiology
Species Specificity
Transgenes
Grant Support
ID/Acronym/Agency:
R01 GM074619/GM/NIGMS NIH HHS; R01 GM074619/GM/NIGMS NIH HHS; R01 GM074619-01A2/GM/NIGMS NIH HHS; R01 GM074619-02/GM/NIGMS NIH HHS; R01 GM074619-03/GM/NIGMS NIH HHS; R01 GM074619-04/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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