Document Detail


High-resolution analysis of a hypervariable region in the human apolipoprotein B gene.
MedLine Citation:
PMID:  2773938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A hypervariable region occurs immediately 3' of the human apolipoprotein B gene. Several allelic variants of this tandemly repeated sequence can be resolved by genomic blotting. Higher resolution among size variants may be obtained by polymerase-chain-reaction amplification of this region followed by electrophoresis in a denaturing acrylamide gel. Fourteen different alleles containing 25-52 repeats of the basic 15-bp unit were distinguished in a population study of 318 unrelated individuals. This approach should be applicable to pedigree and linkage analysis with the apolipoprotein B gene or other tandemly repeated sequence elements.
Authors:
E H Ludwig; W Friedl; B J McCarthy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  45     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-09-22     Completed Date:  1989-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  458-64     Citation Subset:  IM    
Affiliation:
Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140-0608.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Apolipoproteins B / genetics*
Electrophoresis, Polyacrylamide Gel
Gene Amplification
Gene Frequency
Genetic Variation*
Humans
Molecular Sequence Data
Oligonucleotide Probes
Plasmids
Repetitive Sequences, Nucleic Acid*
Grant Support
ID/Acronym/Agency:
HL 38781/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins B; 0/Oligonucleotide Probes
Comments/Corrections

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