Document Detail


High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.
MedLine Citation:
PMID:  6950411     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for high-resolution analysis of the human karyotype by flow cytometry has been developed. Metaphase chromosomes are prepared from short-term peripheral blood cultures, stained with ethidium bromide, and analyzed on a standard fluorescence-activated cell sorter (FACS-II). Flow karyotypes with up to 20 peaks can be obtained with coefficients of variation in the range 1-2%. At this level of resolution the contribution of many of the human chromosomes can be evaluated separately. Significant and reproducible differences between normal individuals have been detected and have been correlated with differences in the centric heterochromatin of certain chromosomes as revealed in their C-banded karyotypes.
Authors:
B D Young; M A Ferguson-Smith; R Sillar; E Boyd
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  78     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7727-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / methods
Cells, Cultured
Chromosome Banding / methods
Chromosomes, Human / ultrastructure*
Flow Cytometry / methods
Humans
Karyotyping / methods
Lymphocytes / ultrastructure*
Comments/Corrections

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