Document Detail


High-resolution analysis by nano-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for the identification of molecular species of phospholipids and their oxidized metabolites.
MedLine Citation:
PMID:  15384179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nano-electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) was applied to identify the molecular species of phosphatidylethanolamine of Caenorhabditis elegans, which has a high concentration of phospholipids with a fatty acyl chain having an odd number of carbon atoms. The molecular species of diacyl phosphatidylethanolamine with one fatty acyl chain having an odd number of carbon atoms and one fatty acyl chain having an even number of carbon atoms was identified separately from alkyl-acyl phosphatidylethanolamine with an alkyl chain having an even number of carbon atoms and a fatty acyl chain having an even number of carbon atoms. Furthermore, nano-ESI-FTICRMS was applied to the direct identification of oxidized phosphatidylcholine from soybean. The mass peaks of individual molecular species of oxidative phosphatidylcholine, such as 34:3 diacyl phosphatidylcholine with peroxide (+2O) (m/z 788.544) or 34:2 diacyl phosphatidylcholine with peroxide (+2O) (m/z 790.560), can be separated from the peaks of the molecular species of the non-oxidative phospholipids. This suggests that the mass peaks with a difference of less than 0.1 mass units in their molecular weight can be separated and that their individual exact molecular compositions can be obtained by the FTICRMS analysis. The high resolution and high accuracy of FTICRMS are very effective in the analysis of molecular species of phospholipids and their derivatives.
Authors:
Mayuko Ishida; Toshiyuki Yamazaki; Toshiaki Houjou; Masayoshi Imagawa; Ayako Harada; Keizou Inoue; Ryo Taguchi
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  18     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2004  
Date Detail:
Created Date:  2004-10-18     Completed Date:  2004-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2486-94     Citation Subset:  IM    
Copyright Information:
2004 John Wiley & Sons, Ltd.
Affiliation:
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya City, Aichi 467, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aerosols / analysis,  chemistry
Animals
Caenorhabditis elegans / metabolism*
Cyclotrons
Nanotechnology / methods
Oxidation-Reduction
Phospholipids / analysis*,  metabolism*
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / methods*
Spectroscopy, Fourier Transform Infrared / methods*
Chemical
Reg. No./Substance:
0/Aerosols; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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