Document Detail


High-resolution analysis of 9p loss in human cancer cells using single nucleotide polymorphism-based mapping arrays.
MedLine Citation:
PMID:  16965958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Single nucleotide polymorphism (SNP) mapping arrays were used to perform DNA copy number analysis of five human cancer cell lines (four malignant mesotheliomas; one non-small cell lung carcinoma) to identify and map the end-points of deletions of 9p. All five cell lines exhibited homozygous deletions encompassing the CDKN2A (alias INK4A/ARF) and CDKN2B loci. The DNA analysis profiles demarcated precisely two different, but overlapping, deletions in each mesothelioma cell line, but the lung cancer cells showed two copies of a single deletion. In the latter cell line, allele analysis revealed that virtually all SNPs for chromosome 9 were homozygous, suggestive of uniparental disomy. These findings demonstrate the utility of SNP-based mapping arrays for high-resolution analysis of genomic imbalances in cancer cells.
Authors:
Jianming Pei; Warren D Kruger; Joseph R Testa
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  170     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-12     Completed Date:  2006-10-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  65-8     Citation Subset:  IM    
Affiliation:
Human Genetics Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Chromosome Deletion*
Chromosomes, Human, Pair 9*
Humans
Multiple Myeloma / genetics*,  pathology
Oligonucleotide Array Sequence Analysis
Polymorphism, Single Nucleotide*
Grant Support
ID/Acronym/Agency:
CA-06927/CA/NCI NIH HHS; CA-45745/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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