Document Detail


High-resolution MRI characterization of human thrombus using a novel fibrin-targeted paramagnetic nanoparticle contrast agent.
MedLine Citation:
PMID:  11108623     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, the sensitivity of a novel fibrin-targeted contrast agent for fibrin detection was defined in vitro on human thrombus. The contrast agent was a lipid-encapsulated perfluorocarbon nanoparticle with numerous Gd-DTPA complexes incorporated into the outer surface. After binding to fibrin clots, scanning electron microscopy of treated clots revealed dense accumulation of nanoparticles on the clot surfaces. Fibrin clots with sizes ranging from 0.5-7.0 mm were imaged at 4.7 T with or without treatment with the targeted contrast agent. Regardless of sizes, untreated clots were not detectable by T(1)-weighted MRI, while targeted contrast agent dramatically improved the detectability of all clots. Decreases in T(1) and T(2) relaxation times (20-40%) were measured relative to the surrounding media and the control clots. These results suggest the potential for sensitive and specific detection of microthrombi that form on the intimal surfaces of unstable atherosclerotic plaque.
Authors:
X Yu; S K Song; J Chen; M J Scott; R J Fuhrhop; C S Hall; P J Gaffney; S A Wickline; G M Lanza
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  44     ISSN:  0740-3194     ISO Abbreviation:  Magn Reson Med     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-09     Completed Date:  2001-01-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  867-72     Citation Subset:  IM    
Affiliation:
Department of Medicine, Cardiovascular Division, Barnes-Jewish Hospital, Washington University School of Medicine, St. Louis, Missouri, USA. xin@cvu.wustl.edu
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / diagnostic use
Biotinylation
Contrast Media*
Drug Evaluation, Preclinical
Fibrin / diagnostic use*,  immunology,  ultrastructure
Gadolinium DTPA / diagnostic use*
Humans
Hydrocarbons, Chlorinated / diagnostic use*
Hydrocarbons, Fluorinated / diagnostic use*
Magnetic Resonance Imaging / methods*
Microscopy, Electron, Scanning
Particle Size
Plasma
Sensitivity and Specificity
Thrombosis / blood,  diagnosis*
Time Factors
Grant Support
ID/Acronym/Agency:
R01 HL-59865/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Contrast Media; 0/Hydrocarbons, Chlorinated; 0/Hydrocarbons, Fluorinated; 0/perfluorodichlorooctane; 80529-93-7/Gadolinium DTPA; 9001-31-4/Fibrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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