Document Detail


High quality human immunoglobulin G purified from Cohn fractions by liquid chromatography.
MedLine Citation:
PMID:  10625871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to obtain intravenous immunoglobulin G (iv IgG) of high quality from F-I+II+III or F-II+III pastes prepared by the Cohn method, we developed a chromatography process using ion exchange gels, Q-Sepharose FF and CM-Sepharose FF, and Sephacryl S-300 gel filtration. Viral inactivation was performed by incubating the preparation with pepsin at pH 4.0 at 35 degrees C for 18 h. The characteristics of 28 batches produced by us were: yield 4.3 +/- 0.2 g/l plasma, i.e., a recovery of 39.1 +/- 1.8%; IgG subclasses distribution: IgG1 = 58.4%, IgG2 = 34.8%, IgG3 = 4.5% and IgG4 = 2. 3%; IgG size distribution was 98.4% monomers, 1.2% dimers and 0.4% polymers and protein aggregates; anticomplement activity was less than 0.5 CH50/mg IgG, and prekallikrein activator activity (PKA) was less than 5 IU/ml. These characteristics satisfied the requirements of the European Pharmacopoea edition, and the regulations of the Brazilian Health Ministry (M.S. Portaria No. 2, 30/10/1998).
Authors:
K Tanaka; E Sawatani; G A Dias; E M Shigueoka; T C Campos; H C Nakao; F Arashiro
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  33     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-06-05     Completed Date:  2000-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  BRAZIL    
Other Details:
Languages:  eng     Pagination:  27-30     Citation Subset:  IM    
Affiliation:
Divisão de Pesquisa e Desenvolvimento Industrial, Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brasil. georgiaandrade@originet.com.br
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid / methods*
Humans
Immunoglobulin G / isolation & purification*
Immunoglobulins, Intravenous / standards*
Reference Standards
Chemical
Reg. No./Substance:
0/Immunoglobulin G; 0/Immunoglobulins, Intravenous

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