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High-purity enrichment of functional cardiovascular cells from human iPS cells.
MedLine Citation:
PMID:  22673369     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: A variety of human inherited heart diseases affect the normal functions of cardiomyocytes (CMs), endothelial cells (ECs) or smooth muscle cells (SMCs). To study human heart disease and generate cardiac cells for basic and translational research, an efficient strategy is needed for production of cardiac lineages from human stem cells. The present study developed a highly reproducible method that can simultaneously enrich a large number of CMs and cardiac SMCs and ECs from human iPS cells with high purity.Methods and ResultsIn this study, human multipotent cardiovascular progenitor (MCP) cells were generated from human iPS cells, followed by selective differentiation of MCPs into CM, ECs, and SMCs. With further fluorescence activated cell sorting (FACS), all three cardiovascular cells could be each enriched with high purity (>90%). These enriched cardiovascular cells exhibited specific gene expression signatures and normal functions when assayed both in vitro and in vivo. Moreover, CMs purified from iPS cells derived from a patient with Leopard Syndrome, a disease characterized by cardiac hypertrophy, showed the expected upregulated expression of genes associated with cardiac hypertrophy. CONCLUSIONS: Overall, our technical advance provides the means for generating a renewable resource of pure human cardiovascular cells that can be used to dissect the mechanism of human inherited heart disease, and for the future development of drug and cell therapeutics for heart disease.
Authors:
Bo Lin; Jong Kim; Yanxin Li; Haiying Pan; Xonia Carvajal-Vergara; Guy Salama; Tao Cheng; Yong Li; Cecilia W Lo; Lei Yang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-6
Journal Detail:
Title:  Cardiovascular research     Volume:  -     ISSN:  1755-3245     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Developmental Biology, University of Pittsburgh School of Medicine, 530 45th Street, Rangos Research Center, Pittsburgh, PA 15201, USA.
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