| High production of D-tagatose by the addition of boric acid. | |
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MedLine Citation:
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PMID: 17583351 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An L-arabinose isomerase mutant enzyme from Geobacillus thermodenitrificans was used to catalyze the isomerization of D-galactose to D-tagatose with boric acid. Maximum production of D-tagatose occurred at pH 8.5-9.0, 60 degrees C, and 0.4 molar ratio of boric acid to D-galactose, and the production increased with increasing enzyme concentration. Under the optimum conditions, the enzyme (10.8 units/mL) converted 300 g/L D-galactose to 230 g/L D-tagatose for 20 h with a yield of 77% (w/w); the production and conversion yield with boric acid were 1.5-fold and 24% higher than without boric acid, respectively. In 24 h, the enzyme produced 370 g/L D-tagatose from 500 g/L D-galactose with boric acid, corresponding to a conversion yield of 74% (w/w) and a production rate of 15.4 g/L.h. The production and yield of D-tagatose obtained in this study are unprecedented. |
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Authors:
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Byung-Chul Lim; Hye-Jung Kim; Deok-Kun Oh |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-21 |
Journal Detail:
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Title: Biotechnology progress Volume: 23 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2007 Jul-Aug |
Date Detail:
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Created Date: 2007-08-03 Completed Date: 2007-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 824-8 Citation Subset: IM |
Affiliation:
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Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-747, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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metabolism Bioreactors Biotechnology / methods* Boric Acids / chemistry* Chromatography, Thin Layer Enzyme Stability Galactose / chemistry Hexoses / biosynthesis*, chemistry Hydrogen-Ion Concentration Industrial Microbiology / methods* Mutation Plasmids / metabolism Recombinant Proteins Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Boric Acids; 0/Hexoses; 0/Recombinant Proteins; 11113-50-1/boric acid; 17598-81-1/tagatose; 26566-61-0/Galactose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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