Document Detail


High production of D-tagatose by the addition of boric acid.
MedLine Citation:
PMID:  17583351     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An L-arabinose isomerase mutant enzyme from Geobacillus thermodenitrificans was used to catalyze the isomerization of D-galactose to D-tagatose with boric acid. Maximum production of D-tagatose occurred at pH 8.5-9.0, 60 degrees C, and 0.4 molar ratio of boric acid to D-galactose, and the production increased with increasing enzyme concentration. Under the optimum conditions, the enzyme (10.8 units/mL) converted 300 g/L D-galactose to 230 g/L D-tagatose for 20 h with a yield of 77% (w/w); the production and conversion yield with boric acid were 1.5-fold and 24% higher than without boric acid, respectively. In 24 h, the enzyme produced 370 g/L D-tagatose from 500 g/L D-galactose with boric acid, corresponding to a conversion yield of 74% (w/w) and a production rate of 15.4 g/L.h. The production and yield of D-tagatose obtained in this study are unprecedented.
Authors:
Byung-Chul Lim; Hye-Jung Kim; Deok-Kun Oh
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-21
Journal Detail:
Title:  Biotechnology progress     Volume:  23     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2007 Jul-Aug
Date Detail:
Created Date:  2007-08-03     Completed Date:  2007-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  824-8     Citation Subset:  IM    
Affiliation:
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-747, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Bacillus / metabolism
Bioreactors
Biotechnology / methods*
Boric Acids / chemistry*
Chromatography, Thin Layer
Enzyme Stability
Galactose / chemistry
Hexoses / biosynthesis*,  chemistry
Hydrogen-Ion Concentration
Industrial Microbiology / methods*
Mutation
Plasmids / metabolism
Recombinant Proteins
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Boric Acids; 0/Hexoses; 0/Recombinant Proteins; 11113-50-1/boric acid; 17598-81-1/tagatose; 26566-61-0/Galactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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