Document Detail


High prevalence and phylogenetic analysis of TT-virus infection in Mongolia.
MedLine Citation:
PMID:  10392725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel DNA virus, TT-virus (TTV), was isolated from a post-transfusion hepatitis patient in Japan. The prevalence of TTV infection was investigated among patients with chronic liver disease and normal alanine aminotransferase (ALT) volunteers as controls in Mongolia. Polymerase chain reaction (PCR) was employed to detect TTV DNA using specific primers derived from open reading frame 1 (ORF1) of the TTV genome. Nucleotide sequences of samples positive for TTV DNA were determined. The sequences were analyzed by a molecular evolutionary method. Fifty (60.2%) hepatitis patients and 12 (42.9%) volunteers were positive for TTV DNA. The serum ALT levels did not differ significantly between patients with single TTV infection and without TTV, HBV and HCV infection. Similarly, the serum ALT levels did not differ significantly between controls with and without TTV infection. Dual infection of TTV with either HBV or HCV did not affect the ALT levels of hepatitis patients. The molecular evolutionary tree showed that TTV was a heterogeneous virus and all strains could be divided into three genotypes in Mongolia. A new genotype was identified that was distinct from those previously reported.
Authors:
H Kato; M Mizokami; T Nakano; Y Kondo; B Dashnyam; T Oyunsuren; R Ueda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virus research     Volume:  60     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-08-11     Completed Date:  1999-08-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  171-9     Citation Subset:  IM    
Affiliation:
Second Department of Medicine, Nagoya City University Medical School, Kawasumi, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB017881;  AB017885;  AB017892;  AB017893;  AB020060;  AB020166;  AB020167;  AB020168;  AB020169;  AB020170;  AB020171;  AB020172;  AB020173;  AB020174;  AB020175;  AB020176;  AB020177;  AB020178;  AB020179;  AB020180;  AB020181;  AB020182;  AB020183;  AB020184;  AB020185;  AF079541;  AF079542;  AF079543
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MeSH Terms
Descriptor/Qualifier:
Adult
Alanine Transaminase / blood
Base Sequence
DNA Virus Infections / epidemiology,  genetics*
DNA Viruses / genetics*
DNA, Viral / chemistry,  genetics
Evolution, Molecular
Female
Hepatitis B / blood
Hepatitis B Surface Antigens / blood
Hepatitis C / blood
Hepatitis C Antibodies / blood
Hepatitis, Viral, Human / epidemiology,  genetics*
Humans
Male
Molecular Sequence Data
Mongolia / epidemiology
Phylogeny*
Prevalence
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Hepatitis B Surface Antigens; 0/Hepatitis C Antibodies; EC 2.6.1.2/Alanine Transaminase

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