Document Detail

High prevalence of Mycobacterium tuberculosis DNA in biopsies from sarcoidosis patients from Catalonia, Spain.
MedLine Citation:
PMID:  16113515     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Sarcoidosis is a systemic granulomatous disease of unknown etiology. The presence of mycobacterial nucleic acid components in patients with sarcoidosis has been demonstrated with varying degrees of success. OBJECTIVES: The aim of this study was to estimate the presence of Mycobacterium tuberculosis DNA in tissues from sarcoidosis patients, in Catalonia, Spain, as well as to assess the long-term clinical course in these patients. METHODS: Fifty-eight paraffin-embedded tissue biopsies corresponding to cases of sarcoidosis (n = 23), lung neoplasm (n = 23), and lung tuberculosis (n = 12) available in 1996 were analyzed in a retrospective study by means of a nested polymerase chain reaction using primers corresponding to the insertion element IS6110 of M. tuberculosis complex. For greater sensitivity, Southern blot hybridization was performed. Clinical data were recorded prior to and after PCR analysis (follow-up reported until 2002). RESULTS: M. tuberculosis DNA was present in 9 out of 23 sarcoidosis biopsies (39%), in 1 out of 23 control patients (4%) (p < 0.01), and in all tissue samples from the 12 control patients with tuberculosis. To date, none of these sarcoidosis patients has developed tuberculosis over a mean (+/-SD) follow-up period of 11 (+/-3.4) years. CONCLUSION: In our setting, M. tuberculosis DNA is present in tissue biopsies of significantly more sarcoidosis patients than controls. However, these results do not demonstrate causality, although they may suggest a link between M. tuberculosis infection and sarcoidosis in some cases. Follow-up of these patients suggests that M. tuberculosis-DNA-positive sarcoidosis patients are not at greater risk of developing tuberculosis than M. tuberculosis-DNA-negative patients.
E Fité; M T Fernández-Figueras; R Prats; M Vaquero; J Morera
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-17
Journal Detail:
Title:  Respiration; international review of thoracic diseases     Volume:  73     ISSN:  0025-7931     ISO Abbreviation:  Respiration     Publication Date:  2006  
Date Detail:
Created Date:  2006-03-01     Completed Date:  2006-06-15     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0137356     Medline TA:  Respiration     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  20-6     Citation Subset:  IM    
Copyright Information:
Copyright 2006 S. Karger AG, Basel.
Servei de Pneumologia, Hospital Universitari Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain.
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MeSH Terms
Antigens, Bacterial / immunology
Case-Control Studies
DNA, Bacterial / analysis*
Genetic Predisposition to Disease
Middle Aged
Mycobacterium tuberculosis / genetics*,  immunology
Sarcoidosis / epidemiology*,  genetics,  immunology,  microbiology*
Spain / epidemiology
Tuberculosis / epidemiology*
Reg. No./Substance:
0/Antigens, Bacterial; 0/DNA, Bacterial
Comment In:
Respiration. 2006;73(1):14-5   [PMID:  16498268 ]

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