Document Detail

High pressure treatment as a tool for engineering of enzymatic reactions in cellulosic fibres.
MedLine Citation:
PMID:  22244958     Owner:  NLM     Status:  Publisher    
Ultrahigh hydrostatic pressure (UHP) was applied to enhance the enzymatic hydrolysis of xylan in cellulosic fibers. The pretreatment improved xylan accessibility in the fiber cell wall and a 5-10-fold increase in the initial hydrolysis rate of xylan by xylanase was observed when Eucalyptus globulus kraft bleached pulp was pretreated at hydrostatic pressures of 300-400MPa for 15-45min. The rate and the extent of hydrolysis can be controlled by varying the pressure and the exposure time of UHP treatment prior to enzymatic hydrolysis. Sequential UHP pretreatment and hydrolysis by xylanase disaggregated the hydrated cellulose fibrils and led to the gelation of cell wall regions accessible to enzymatic attack. UHP could therefore become a tool for the engineering of enzymatic reactions within cellulosic fibers to obtain products with tailored properties.
Sandra C T Oliveira; Andreia B Figueiredo; Dmitry V Evtuguin; Jorge A Saraiva
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-24
Journal Detail:
Title:  Bioresource technology     Volume:  -     ISSN:  1873-2976     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
CICECO, Chemistry Department, University of Aveiro, Campus de Santiago, P-3810-193 Aveiro, Portugal.
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