Document Detail

High pressure thermal inactivation kinetics of a plasmin system.
MedLine Citation:
PMID:  15328256     Owner:  NLM     Status:  MEDLINE    
A crude plasmin extract was prepared from milk by ultracentrifugation and was partially purified using ammonium sulfate precipitation. Isothermal and high-pressure inactivation of this plasmin system at pH 6.7 could be described by a first-order kinetic model. As expected, the plasmin system displayed a high thermostability. High-pressure treatments were conducted in the 300- to 800-MPa pressure range, combined with temperatures from 25 to 65 degrees C. The plasmin system was very pressure stable at room temperature, but inactivation occurred with combined high-pressure/temperature-treatments. The influence of temperature at different constant pressures on the inactivation rate constant was quantified using the Arrhenius equation. At all temperatures studied, a synergistic effect of temperature and high pressure was observed in the 300- to 600-MPa pressure range. However, an antagonistic effect of temperature and pressure appeared at pressures above 600 MPa.
D Borda; Indrawati; C Smout; A Van Loey; M Hendrickx
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  87     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-25     Completed Date:  2004-11-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2351-8     Citation Subset:  IM    
Department of Food Bioengineering, Faculty of Food Science and Engineering, University Dunarea de Jos, 800201, Galati, Romania.
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MeSH Terms
Enzyme Activation
Enzyme Stability
Fibrinolysin / isolation & purification,  metabolism*
Hot Temperature*
Hydrogen-Ion Concentration
Milk / enzymology
Reg. No./Substance:

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