| High-pressure refolding of human vascular endothelial growth factor (VEGF) recombinantly expressed in bacterial inclusion bodies: Refolding optimization, and feasibility assessment. | |
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MedLine Citation:
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PMID: 21608142 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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High-pressure has been established as an effective technique for refolding proteins at high concentrations. In this study, high hydrostatic pressure (1-3 kbar) was utilized to refold a homodimeric protein from inclusion bodies and the process was evaluated for large-scale manufacturing feasibility. This research focused on increasing protein concentration while maximizing yield and product quality. Refolding yields of 29-42% were achieved in the absence of urea at 2 kbar and at a protein concentration of 6 g/L. Optimization of the refolding buffer composition via multivariate design of experiments and other process parameters such as refolding pressure, gas sparging, and time under pressure are discussed. Although high-pressure refolding can be considered a viable technology for manufacturing if the gains are clearly identified, in this particular case, the benefits that the high-pressure technology offers do not compensate for the drawbacks of implementing new equipment in an existing facility, and unknown impact of scale-up for this molecule. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011. |
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Authors:
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Amber Cothran; Richard J St John; Charles H Schmelzer; Shelly A Pizarro |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-2 |
Journal Detail:
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Title: Biotechnology progress Volume: - ISSN: 1520-6033 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 American Institute of Chemical Engineers (AIChE). |
Affiliation:
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Genentech, Pharma Technical Development, 1 DNA Way, South San Francisco, CA 94080. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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