Document Detail


High-pressure macromolecular crystallography and NMR: status, achievements and prospects.
MedLine Citation:
PMID:  22959123     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Biomacromolecules are thermodynamic entities that exist in general as an equilibrium mixture of the basic folded state and various higher-energy substates including all functionally relevant ones. Under physiological conditions, however, the higher-energy substates are usually undetectable on spectroscopy, as their equilibrium populations are extremely low. Hydrostatic pressure gives a general solution to this problem. As proteins generally have smaller partial molar volumes in higher-energy states than in the basic folded state, pressure can shift the equilibrium toward the former substantially, and allows their direct detection and analysis with X-ray crystallography or NMR spectroscopy at elevated pressures. These techniques are now mature, and their status and selected applications are presented with future prospects.
Authors:
Roger Fourme; Eric Girard; Kazuyuki Akasaka
Related Documents :
23597043 - Recent progress on the discovery of non-peptidic direct renin inhibitors for the clinic...
17959963 - In vitro evaluation of a micro-doppler catheter for detection and aspiration of venous ...
22174583 - Arterial stiffness, central hemodynamics, and cardiovascular risk in hypertension.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-4
Journal Detail:
Title:  Current opinion in structural biology     Volume:  -     ISSN:  1879-033X     ISO Abbreviation:  Curr. Opin. Struct. Biol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107784     Medline TA:  Curr Opin Struct Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Synchrotron Soleil, BP48 Saint Aubin, 91192 Gif sur Yvette, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Renal transplant improves pulmonary hypertension in patients with end stage renal disease.
Next Document:  Increased Sensorimotor Gating in Recreational and Dependent Cocaine Users Is Modulated by Craving an...