| High-pressure macromolecular crystallography and NMR: status, achievements and prospects. | |
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MedLine Citation:
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PMID: 22959123 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Biomacromolecules are thermodynamic entities that exist in general as an equilibrium mixture of the basic folded state and various higher-energy substates including all functionally relevant ones. Under physiological conditions, however, the higher-energy substates are usually undetectable on spectroscopy, as their equilibrium populations are extremely low. Hydrostatic pressure gives a general solution to this problem. As proteins generally have smaller partial molar volumes in higher-energy states than in the basic folded state, pressure can shift the equilibrium toward the former substantially, and allows their direct detection and analysis with X-ray crystallography or NMR spectroscopy at elevated pressures. These techniques are now mature, and their status and selected applications are presented with future prospects. |
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Authors:
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Roger Fourme; Eric Girard; Kazuyuki Akasaka |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-4 |
Journal Detail:
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Title: Current opinion in structural biology Volume: - ISSN: 1879-033X ISO Abbreviation: Curr. Opin. Struct. Biol. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107784 Medline TA: Curr Opin Struct Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Synchrotron Soleil, BP48 Saint Aubin, 91192 Gif sur Yvette, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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