Document Detail

High-pressure macromolecular crystallography (HPMX): status and prospects.
MedLine Citation:
PMID:  16487756     Owner:  NLM     Status:  MEDLINE    
Recent technical developments, achievements and prospects of high-pressure (HP) macromolecular crystallography (MX) are reviewed. Technical difficulties associated with this technique have been essentially solved by combining synchrotron radiation of ultra-short wavelength, large-aperture diamond anvil cells and new sample-mounting techniques. The quality of diffraction data collected at HP can now meet standards of conventional MX. The exploitation of the potential of the combination of X-ray diffraction and high-pressure perturbation is progressing well. The ability of pressure to shift the population distribution of conformers in solution, which is exploited in particular by NMR, can also be used in the crystalline state with specific advantages. HPMX has indeed bright prospects, in particular to elucidate the structure of higher-energy conformers that are often of high biological significance. Furthermore, HPMX may be of interest for conventional crystallographic studies, as pressure is a fairly general tool to improve order in pre-existing crystals with minimal perturbation of the native structure.
Roger Fourme; Eric Girard; Richard Kahn; Anne-Claire Dhaussy; Mohamed Mezouar; Nathalie Colloc'h; Isabella Ascone
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Publication Detail:
Type:  Journal Article; Review     Date:  2006-01-30
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1764     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-04-07     Completed Date:  2006-06-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  384-90     Citation Subset:  IM    
Synchrotron-SOLEIL, BP48 Saint Aubin, 91192 Gif sur Yvette, France.
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MeSH Terms
Crystallography, X-Ray / methods*
Protein Conformation
Proteins / chemistry*
X-Ray Diffraction
Reg. No./Substance:

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