| High pressure liquid chromatography methods for separation of omega- and (omega-1)-hydroxy fatty acids: their applications to microsomal fatty acid omega-oxidation. | |
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MedLine Citation:
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PMID: 3133959 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fatty acids (C12-C18) and their omega- and (omega-1)-hydroxy derivatives, when converted to p-bromophenacyl (PBP) esters, can be completely separated from one another by high pressure liquid chromatography (HPLC) on a silicic acid column using 0.5% (v/v) isopropanol in n-hexane. In this system, fatty acid PBP esters are eluted at the solvent front, whereas the retention times of the omega- and (omega-1)-hydroxy derivatives are 14-20 and 24-29 min, respectively. The PBP esters can also be separated by reverse phase HPLC on a muBondapak C18 column, a method which has been developed by Fan et al. (Fan, L. L., Masters, B. S. S., and Prough, R. A. (1976) Anal. Biochem. 71, 265-272) for separation of methyl esters of fatty acids and their omega- and (omega-1)-hydroxy derivatives. In the latter method, however, the retention times of omega- and (omega-1)-hydroxy derivatives are only about 2 min apart and an increase in the solvent polarity is needed for elution of the esters of unmodified fatty acids. Fatty acid PBP esters, however, can be obtained as independent peaks which are not disturbed by the solvent front. An application of the former method to measure fatty acid omega oxidation by liver microsomes and by a reconstituted monooxygenase system containing purified cytochrome P-450 is described. |
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Authors:
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T Aoyama; R Sato |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 170 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1988 Apr |
Date Detail:
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Created Date: 1988-07-29 Completed Date: 1988-07-29 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 73-82 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkane 1-Monooxygenase Animals Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System / metabolism Hydroxy Acids / analysis* Microsomes, Liver / enzymology* Mixed Function Oxygenases / metabolism Oxidation-Reduction Oxygenases / analysis Palmitic Acid Palmitic Acids / metabolism Phenols / analysis Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Hydroxy Acids; 0/Palmitic Acids; 0/Phenols; 106-41-2/4-bromophenol; 57-10-3/Palmitic Acid; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.13.-/Oxygenases; EC 1.14.15.3/Alkane 1-Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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