Document Detail


High pressure liquid chromatography methods for separation of omega- and (omega-1)-hydroxy fatty acids: their applications to microsomal fatty acid omega-oxidation.
MedLine Citation:
PMID:  3133959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acids (C12-C18) and their omega- and (omega-1)-hydroxy derivatives, when converted to p-bromophenacyl (PBP) esters, can be completely separated from one another by high pressure liquid chromatography (HPLC) on a silicic acid column using 0.5% (v/v) isopropanol in n-hexane. In this system, fatty acid PBP esters are eluted at the solvent front, whereas the retention times of the omega- and (omega-1)-hydroxy derivatives are 14-20 and 24-29 min, respectively. The PBP esters can also be separated by reverse phase HPLC on a muBondapak C18 column, a method which has been developed by Fan et al. (Fan, L. L., Masters, B. S. S., and Prough, R. A. (1976) Anal. Biochem. 71, 265-272) for separation of methyl esters of fatty acids and their omega- and (omega-1)-hydroxy derivatives. In the latter method, however, the retention times of omega- and (omega-1)-hydroxy derivatives are only about 2 min apart and an increase in the solvent polarity is needed for elution of the esters of unmodified fatty acids. Fatty acid PBP esters, however, can be obtained as independent peaks which are not disturbed by the solvent front. An application of the former method to measure fatty acid omega oxidation by liver microsomes and by a reconstituted monooxygenase system containing purified cytochrome P-450 is described.
Authors:
T Aoyama; R Sato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  170     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-07-29     Completed Date:  1988-07-29     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  73-82     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892.
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MeSH Terms
Descriptor/Qualifier:
Alkane 1-Monooxygenase
Animals
Chromatography, High Pressure Liquid
Cytochrome P-450 Enzyme System / metabolism
Hydroxy Acids / analysis*
Microsomes, Liver / enzymology*
Mixed Function Oxygenases / metabolism
Oxidation-Reduction
Oxygenases / analysis
Palmitic Acid
Palmitic Acids / metabolism
Phenols / analysis
Rabbits
Chemical
Reg. No./Substance:
0/Hydroxy Acids; 0/Palmitic Acids; 0/Phenols; 106-41-2/4-bromophenol; 57-10-3/Palmitic Acid; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.13.-/Oxygenases; EC 1.14.15.3/Alkane 1-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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