Document Detail

High pressure induces G2 arrest in murine erythroleukemia cells.
MedLine Citation:
PMID:  9504413     Owner:  NLM     Status:  MEDLINE    
The effect of high pressure on proliferation and cell cycle progression was examined using murine erythroleukemia (MEL) cells. The MEL cells were exposed to high pressures (0.1-130 MPa) and then cultured for 5 days at atmospheric pressure. The proliferation of MEL cells was unaffected up to 60 MPa, but was suppressed at 80-110 MPa. Above 120 MPa, the cells were fragmented. The cell cycle analysis of 80 MPa-treated MEL cells showed that the cells in S phase are most sensitive to high pressure and they arrest in G2 phase. Interestingly, G2-arrested cells reinitiated DNA synthesis, resulting in giant cells with high DNA contents. Furthermore, when such G2-arrested cells were exposed to caffeine, premature mitosis, characterized by chromosome pulverization, was observed. These results suggest that the suppression of proliferation in high-pressure-treated MEL cells is associated with G2 arrest following S phase delay. Thus, it seems valuable to apply high pressure to the investigation of the cell cycle.
M Matsumoto; T Yamaguchi; Y Fukumaki; R Yasunaga; S Terada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry     Volume:  123     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-03-23     Completed Date:  1998-03-23     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  87-93     Citation Subset:  IM    
Department of Chemistry, Faculty of Science, Fukuoka University.
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MeSH Terms
Adenine / analogs & derivatives,  pharmacology
Caffeine / pharmacology
Cell Cycle / drug effects
Cell Division*
G2 Phase*
Leukemia, Erythroblastic, Acute
Mitotic Index
Tumor Cells, Cultured
Reg. No./Substance:
58-08-2/Caffeine; 73-24-5/Adenine; 938-55-6/N(6),N(6)-dimethyladenine

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