Document Detail


High-pressure freezing of tissue obtained by fine-needle biopsy.
MedLine Citation:
PMID:  8805825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-pressure freezing (HPF) permits adequate cryoimmobilization (without detectable ice crystals after freeze-substitution) of biological tissue up to a thickness of about 200 microns. Until now the preparation of tissue prior to freezing has been unsatisfactory: sizing of the tissue to the required dimensions takes minutes, during which structural alterations must occur. We demonstrate that the use of a fine-needle biopsy technique minimizes tissue damage and guarantees sample dimensions close to the optimal thickness for HPF. The tissue cores can be cryoimmobilized within 40 s of excision.
Authors:
H Hohenberg; M Tobler; M Müller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microscopy     Volume:  183     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-10-17     Completed Date:  1996-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  133-9     Citation Subset:  IM    
Affiliation:
Laboratory for Electron Microscopy I, ETH Zürich, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopsy, Needle*
Freezing*
Liver / pathology
Myocardium / pathology
Rats

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