Document Detail


High power light emitting diode based setup for photobleaching fluorescent impurities.
MedLine Citation:
PMID:  19191468     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Single molecule fluorescence experiments, with their associated low signals, require very low background fluorescence in the sample. Even high purity liquids will often possess large numbers of fluorescent impurities that are difficult to completely remove through standard purification techniques such as distillation and recrystallization. We have constructed a simple setup in which such impurities can be photobleached before final sample preparation. The instrument consists of high power light emitting diodes, and it delivers almost 10 W of light to the sample without the heating associated with more conventional light sources or the cost and safety concerns associated with a high power laser.
Authors:
Tobias K Herman; Stephan A Mackowiak; Laura J Kaufman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Review of scientific instruments     Volume:  80     ISSN:  1089-7623     ISO Abbreviation:  Rev Sci Instrum     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-02-04     Completed Date:  2009-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0405571     Medline TA:  Rev Sci Instrum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  016107     Citation Subset:  -    
Affiliation:
Department of Chemistry, Columbia University, New York, New York 10027, USA.
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