| High performance stationary phases for planar chromatography. | |
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MedLine Citation:
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PMID: 21056422 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The kinetic performance of stabilized particle layers, particle membranes, and thin films for thin-layer chromatography is reviewed with a focus on how layer characteristics and experimental conditions affect the observed plate height. Forced flow and pressurized planar electrochromatography are identified as the best candidates to overcome the limited performance achieved by capillary flow for stabilized particle layers. For conventional and high performance plates band broadening is dominated by molecular diffusion at low mobile phase velocities typical of capillary flow systems and by mass transfer with a significant contribution from flow anisotropy at higher flow rates typical of forced flow systems. There are few possible changes to the structure of stabilized particle layers that would significantly improve their performance for capillary flow systems while for forced flow a number of avenues for further study are identified. New media for ultra thin-layer chromatography shows encouraging possibilities for miniaturized high performance systems but the realization of their true performance requires improvements in instrumentation for sample application and detection. |
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Authors:
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Salwa K Poole; Colin F Poole |
Publication Detail:
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Type: Journal Article Date: 2010-10-23 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1218 ISSN: 1873-3778 ISO Abbreviation: J Chromatogr A Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 2648-60 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Detroit District Laboratory, US Food and Drug Administration, 300 River Place, Suite 5900, Detroit, MI 48207, USA. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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