Document Detail


High-performance liquid chromatography of phospholipids: quantitation by phosphate analysis.
MedLine Citation:
PMID:  6670745     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Quantitation of individual phospholipids separated by HPLC from tissue extracts by colorimetric analysis of phosphate was investigated. Elution of inorganic phosphate and breakthrough of lecithin were determined using radioisotopes. A substance which interfered with sample phosphate determinations was found in the column eluant, and a method to minimize its effect was developed. This method allows accurate quantitation of individual phospholipids present at a minimum of 20 nmol phosphate.
Authors:
J R Yandrasitz; G Berry; S Segal
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  135     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-04-06     Completed Date:  1984-04-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  239-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Chemistry
Chromatography, High Pressure Liquid
Liver / analysis
Nerve Endings / analysis
Phosphates / isolation & purification*
Phospholipids / isolation & purification*
Rats
Synaptosomes / analysis
Grant Support
ID/Acronym/Agency:
HD 07107/HD/NICHD NIH HHS; HD 08536/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Phosphates; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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