| High-performance liquid chromatography of phospholipids: quantitation by phosphate analysis. | |
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MedLine Citation:
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PMID: 6670745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantitation of individual phospholipids separated by HPLC from tissue extracts by colorimetric analysis of phosphate was investigated. Elution of inorganic phosphate and breakthrough of lecithin were determined using radioisotopes. A substance which interfered with sample phosphate determinations was found in the column eluant, and a method to minimize its effect was developed. This method allows accurate quantitation of individual phospholipids present at a minimum of 20 nmol phosphate. |
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Authors:
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J R Yandrasitz; G Berry; S Segal |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 135 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1983 Nov |
Date Detail:
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Created Date: 1984-04-06 Completed Date: 1984-04-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 239-43 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry Chromatography, High Pressure Liquid Liver / analysis Nerve Endings / analysis Phosphates / isolation & purification* Phospholipids / isolation & purification* Rats Synaptosomes / analysis |
| Grant Support | |
ID/Acronym/Agency:
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HD 07107/HD/NICHD NIH HHS; HD 08536/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphates; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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