Document Detail

High performance liquid chromatography-electrospray ionization mass spectrometric determination of balofloxacin in human plasma and its pharmacokinetics.
MedLine Citation:
PMID:  17161665     Owner:  NLM     Status:  MEDLINE    
A selective and sensitive high performance liquid chromatography-electrospray ionisation-mass spectrometry method has been developed for the determination of balofloxacin (BLFX) in human plasma. The sample preparation was a liquid-liquid extraction, and chromatographic separation was achieved with an Agilent ZORBAX 300SB C18 2.1 mm x 150 mm column using a mobile phase comprised of methanol-water (10 mM CH(3)COONH(4), pH 3.0)=40:60 (v/v). Standard curves were linear (r=0.9992) over the concentration range of 0.03-3 microg/ml and had good accuracy and precision. The within- and between-batch precisions were within 10% relative standard deviation (R.S.D.). The limit of detection (LOD) was 0.02 microg/ml. The validated HPLC-electrospray ionization (ESI)-MS method has been used successfully to study balofloxacin pharmacokinetics in healthy volunteers.
Zuwei Bian; Yuan Tian; Zunjian Zhang; Fengguo Xu; Jinheng Li; Xiaomei Cao
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Publication Detail:
Type:  Journal Article     Date:  2006-12-11
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  850     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-07-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  68-73     Citation Subset:  IM    
Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, PR China.
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MeSH Terms
Anti-Bacterial Agents / blood*,  pharmacokinetics
Chromatography, High Pressure Liquid / methods*
Fluoroquinolones / blood*,  pharmacokinetics
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / methods*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fluoroquinolones; 127294-70-6/balofloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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