| High performance liquid chromatography-electrospray ionization mass spectrometric determination of balofloxacin in human plasma and its pharmacokinetics. | |
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MedLine Citation:
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PMID: 17161665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A selective and sensitive high performance liquid chromatography-electrospray ionisation-mass spectrometry method has been developed for the determination of balofloxacin (BLFX) in human plasma. The sample preparation was a liquid-liquid extraction, and chromatographic separation was achieved with an Agilent ZORBAX 300SB C18 2.1 mm x 150 mm column using a mobile phase comprised of methanol-water (10 mM CH(3)COONH(4), pH 3.0)=40:60 (v/v). Standard curves were linear (r=0.9992) over the concentration range of 0.03-3 microg/ml and had good accuracy and precision. The within- and between-batch precisions were within 10% relative standard deviation (R.S.D.). The limit of detection (LOD) was 0.02 microg/ml. The validated HPLC-electrospray ionization (ESI)-MS method has been used successfully to study balofloxacin pharmacokinetics in healthy volunteers. |
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Authors:
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Zuwei Bian; Yuan Tian; Zunjian Zhang; Fengguo Xu; Jinheng Li; Xiaomei Cao |
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Publication Detail:
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Type: Journal Article Date: 2006-12-11 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 850 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-04-30 Completed Date: 2007-07-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 68-73 Citation Subset: IM |
Affiliation:
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Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anti-Bacterial Agents / blood*, pharmacokinetics Chromatography, High Pressure Liquid / methods* Female Fluoroquinolones / blood*, pharmacokinetics Humans Male Reference Standards Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Fluoroquinolones; 127294-70-6/balofloxacin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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