Document Detail

High-performance liquid chromatography (HPLC) determination of inosine, a potential biomarker for initial cardiac ischaemia, using isolated mouse hearts.
MedLine Citation:
PMID:  16966161     Owner:  NLM     Status:  MEDLINE    
Each year in the USA approximately 7-8 million patients with non-traumatic chest pain come to hospital emergency rooms. It is estimated that approximately 2-5% of these patients are experiencing cardiac ischaemia, but due to the shortcomings of the available testing methods they are incorrectly diagnosed and discharged without appropriate therapy having been provided. Preliminary data with a globally ischaemic mouse heart model has demonstrated that endogenous inosine might be a potential biomarker of initial cardiac ischaemia before cardiac tissue necrosis. A high-performance liquid chromatographic diode array detection (HPLC-DAD) method was utilized for the detection and quantification of inosine in Krebs-Henseleit (Krebs) buffer solution perfusing from surgically removed and isolated mouse hearts undergoing global cardiac ischaemia. A C18 column at a flow rate of 0.6 ml min-1 with an aqueous mobile phase of trifluoroacetic acid (0.05% trifluoroacetic acid in deionized water, pH 2.2, v/v) and methanol gradient was used for component separation. The assay detection limit for inosine in Krebs buffer solution was 500 ng ml-1 using a 100-microl neat injection. The HPLC results were used to determine total cardiac effluxed inosine into the Krebs effluent for each mouse during oxidative stress and compared with the per cent cardiac ventricular functional recovery rate to determine if a relationship exists amongst this cardiovascular parameter during periods of cardiac oxidative stress.
D Farthing; L Xi; L Gehr; D Sica; T Larus; H T Karnes
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals     Volume:  11     ISSN:  1354-750X     ISO Abbreviation:  Biomarkers     Publication Date:    2006 Sep-Oct
Date Detail:
Created Date:  2006-09-12     Completed Date:  2006-11-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9606000     Medline TA:  Biomarkers     Country:  England    
Other Details:
Languages:  eng     Pagination:  449-59     Citation Subset:  IM    
Department of Cardiology, VCU Medical Center, Virginia Commonwealth UNiversity, Richmond, VA, USA.
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MeSH Terms
Biological Markers / metabolism*
Body Weight
Chromatography, High Pressure Liquid / methods*
Free Radicals / metabolism
Inosine / metabolism*
Mice, Inbred ICR
Myocardial Ischemia / metabolism*
Myocardium / metabolism
Organ Size
Reproducibility of Results
Reg. No./Substance:
0/Biological Markers; 0/Free Radicals; 58-63-9/Inosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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