| High-performance liquid chromatographic measurement of cerebrospinal fluid tetrahydrobiopterin, neopterin, homovanillic acid and 5-hydroxindoleacetic acid in neurological diseases. | |
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MedLine Citation:
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PMID: 7524948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cerebrospinal fluid (CSF) samples from patients with a variety of neurological disorders were assayed to determine the concentrations of tetrahydrobiopterin (BH4), the active cofactor of hydroxylases. Dihydroneopterin (NH2) and neopterin (N), which are linked with BH4 synthesis and are inflammatory biochemical markers, were also measured simultaneously in a number of patients. 5-Hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA), the main products of serotonin and dopamine breakdown, were analyzed in parallel whenever possible. As BH4 and NH2 are difficult to analyze owing to their instability, CSF samples were collected under special conditions to preserve the reduced BH4 and NH2. Liquid chromatographic assays and detection of the various substances measured also required particular precautions. BH4 concentrations were elevated in patients with neurological disorders such as syphilis and lupus-like disease and especially in an AIDS patient with neurological complications with an increased N/BH4 ratio. |
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Authors:
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M Candito; T Nagatsu; P Chambon; M Chatel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 657 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-12-06 Completed Date: 1994-12-06 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 61-6 Citation Subset: IM; X |
Affiliation:
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Laboratoire de Biochimie, Hôpital Pasteur, Nice, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acquired Immunodeficiency Syndrome
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cerebrospinal fluid Adult Aged Aged, 80 and over Biopterin / analogs & derivatives*, cerebrospinal fluid Chromatography, High Pressure Liquid / methods*, statistics & numerical data Dopamine / metabolism Drug Stability Female Homovanillic Acid / cerebrospinal fluid* Humans Hydroxyindoleacetic Acid / cerebrospinal fluid* Male Middle Aged Neopterin Nervous System Diseases / cerebrospinal fluid* Reproducibility of Results Serotonin / metabolism |
| Chemical | |
Reg. No./Substance:
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17528-72-2/5,6,7,8-tetrahydrobiopterin; 22150-76-1/Biopterin; 306-08-1/Homovanillic Acid; 50-67-9/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; 670-65-5/Neopterin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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