Document Detail

High-performance liquid chromatographic measurement of cerebrospinal fluid tetrahydrobiopterin, neopterin, homovanillic acid and 5-hydroxindoleacetic acid in neurological diseases.
MedLine Citation:
PMID:  7524948     Owner:  NLM     Status:  MEDLINE    
Cerebrospinal fluid (CSF) samples from patients with a variety of neurological disorders were assayed to determine the concentrations of tetrahydrobiopterin (BH4), the active cofactor of hydroxylases. Dihydroneopterin (NH2) and neopterin (N), which are linked with BH4 synthesis and are inflammatory biochemical markers, were also measured simultaneously in a number of patients. 5-Hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA), the main products of serotonin and dopamine breakdown, were analyzed in parallel whenever possible. As BH4 and NH2 are difficult to analyze owing to their instability, CSF samples were collected under special conditions to preserve the reduced BH4 and NH2. Liquid chromatographic assays and detection of the various substances measured also required particular precautions. BH4 concentrations were elevated in patients with neurological disorders such as syphilis and lupus-like disease and especially in an AIDS patient with neurological complications with an increased N/BH4 ratio.
M Candito; T Nagatsu; P Chambon; M Chatel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  657     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-12-06     Completed Date:  1994-12-06     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  61-6     Citation Subset:  IM; X    
Laboratoire de Biochimie, Hôpital Pasteur, Nice, France.
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MeSH Terms
Acquired Immunodeficiency Syndrome / cerebrospinal fluid
Aged, 80 and over
Biopterin / analogs & derivatives*,  cerebrospinal fluid
Chromatography, High Pressure Liquid / methods*,  statistics & numerical data
Dopamine / metabolism
Drug Stability
Homovanillic Acid / cerebrospinal fluid*
Hydroxyindoleacetic Acid / cerebrospinal fluid*
Middle Aged
Nervous System Diseases / cerebrospinal fluid*
Reproducibility of Results
Serotonin / metabolism
Reg. No./Substance:
17528-72-2/5,6,7,8-tetrahydrobiopterin; 22150-76-1/Biopterin; 306-08-1/Homovanillic Acid; 50-67-9/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; 670-65-5/Neopterin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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