Document Detail


High-performance liquid chromatographic determination of vancomycin in rabbit serum, vitreous and aqueous humour after intravitreal injection of the drug.
MedLine Citation:
PMID:  8919668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high-performance liquid chromatographic method for the determination of vancomycin in rabbit serum, vitreous and aqueous humour has been developed. No clean-up step was necessary for vitreous and aqueous humour samples. For serum samples liquid-liquid and solid-phase extraction were tested and the best results were achieved using C18 cartridges. The extracts were analyzed on a C18 reversed-phase column, using a mixture of 0.05 M phosphate buffer (pH 4) with 10% of acetonitrile as mobile phase. The detection was carried out at 198 nm, which allows higher sensitivity. The average quantitation limit obtained was 0.03 micrograms/ml. The method has been applied to the study of the residual quantities of vancomycin in serum and rabbit eyes after intravitreal administration of the drug in endophthalmitis treatment.
Authors:
M J Del Nozal; J L Bernal; A Pampliega; P Marinero; M I López; R Coco
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  727     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-12-23     Completed Date:  1996-12-23     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  231-8     Citation Subset:  IM    
Affiliation:
Department of Analytical Chemistry, University de Valladolid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / administration & dosage,  blood,  metabolism*
Aqueous Humor / metabolism*
Chromatography, High Pressure Liquid
Hydrogen-Ion Concentration
Rabbits
Temperature
Vancomycin / administration & dosage,  blood,  metabolism*
Vitreous Body / metabolism*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 1404-90-6/Vancomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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