| High-performance liquid chromatographic determination of tramadol in human plasma. | |
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MedLine Citation:
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PMID: 8798927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tramadol has been determined in human plasma samples using a sensitive high-performance liquid chromatographic method. The plasma samples were extracted with tert.-butylmethyl ether in one-step liquid-liquid extraction (recovery 86%) and analyses of the extracts were performed on reversed-phase silica gel using ion-pair chromatography (verapamil as an internal standard) and fluorescence detection. The method was applied to the determination of tramadol levels in twelve healthy volunteers after oral administration of 100 mg of tramadol in capsules of Protradon and Tramal. |
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Authors:
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M Nobilis; J Pastera; P Anzenbacher; D Svoboda; J Kopecký; F Perlík |
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Publication Detail:
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Type: Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 681 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1996 May |
Date Detail:
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Created Date: 1997-02-05 Completed Date: 1997-02-05 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 177-83 Citation Subset: IM |
Affiliation:
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Institute of Experimental Biopharmaceutics, Joint Research Centre of the Academy of Sciences of the Czech Republic, Hradec Králové, Czech Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Adult Analgesics, Opioid / administration & dosage, blood*, chemistry, pharmacokinetics* Calibration Capsules Chromatography, High Pressure Liquid Cross-Over Studies Female Humans Linear Models Male Reference Standards Reproducibility of Results Spectrophotometry, Ultraviolet Therapeutic Equivalency Tramadol / administration & dosage, blood*, chemistry, pharmacokinetics* Verapamil / blood |
| Chemical | |
Reg. No./Substance:
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0/Analgesics, Opioid; 0/Capsules; 27203-92-5/Tramadol; 52-53-9/Verapamil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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