| High performance liquid chromatographic determination of methacrylate in blood serum. | |
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MedLine Citation:
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PMID: 8130633 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methacrylic acid is a hydrolytic breakdown product of methylmethacrylate which is widely used in orthopaedic surgery, dentistry and in the chemical industry for fabrication of acrylic resins. Information on the toxicity of methacrylic acid and its metabolism is limited. To facilitate studies in this field we developed a liquid chromatographic procedure allowing determination of methacrylate in blood serum. The procedure was based on reversed-phase HPLC using a gradient elution with UV detection at 210 or 240 nm. The method was linear up to 5 mM concentration with a detection limit of 0.5 microM. The procedure was applied to determination of methacrylate in rat blood serum after administration of sodium methacrylate solution into the stomach. A peak of this compound was demonstrated in the chromatogram 10 min after administration which disappeared after 60 min, confirming suitability of the method for biological applications. |
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Authors:
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Z Bereznowski; M Marlewski; R T Smolenski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 8 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 1994 Jan-Feb |
Date Detail:
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Created Date: 1994-04-21 Completed Date: 1994-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 42-4 Citation Subset: IM |
Affiliation:
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Department of Prosthetic Dentistry, Academic Medical School of Gdansk, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Chemical Analysis Chromatography, High Pressure Liquid / methods* Methacrylates / analysis* Rats |
| Chemical | |
Reg. No./Substance:
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0/Methacrylates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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