Document Detail

High performance liquid chromatographic determination of aclacinomycin A and its related compounds. I. Normal phase HPLC for monitoring fermentation and purification processes.
MedLine Citation:
PMID:  6941957     Owner:  NLM     Status:  MEDLINE    
A normal phase high performance liquid chromatographic method is presented for the separation and quantitative determination of aclacinomycin A (ACM) and its related compounds classified as anthracycline antibiotics. The sensitive, reliable and reproducible chromatography was achieved on a mu-Porasil column with a mobile phase consisting of chloroform-methanol-acetic acid-water-triethylamine (68:20:10:2:0.01, v/v). By use of MA144 M1, one of the ACM analogues, as an internal standard, selectivity of the HPLC method could be retained by virtue of the adjustment of the detecting wavelength to 436 nm. The HPLC method was applicable to monitoring the fermentation and purification processes.
T Ogasawara; S Goto; S Mori; T Oki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of antibiotics     Volume:  34     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-09-25     Completed Date:  1981-09-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  47-51     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid / methods
Naphthacenes / analysis,  biosynthesis,  isolation & purification
Streptomyces / metabolism
Reg. No./Substance:
0/Naphthacenes; 57576-44-0/Aclarubicin

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