Document Detail


High performance liquid chromatographic determination of aclacinomycin A and its related compounds. I. Normal phase HPLC for monitoring fermentation and purification processes.
MedLine Citation:
PMID:  6941957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A normal phase high performance liquid chromatographic method is presented for the separation and quantitative determination of aclacinomycin A (ACM) and its related compounds classified as anthracycline antibiotics. The sensitive, reliable and reproducible chromatography was achieved on a mu-Porasil column with a mobile phase consisting of chloroform-methanol-acetic acid-water-triethylamine (68:20:10:2:0.01, v/v). By use of MA144 M1, one of the ACM analogues, as an internal standard, selectivity of the HPLC method could be retained by virtue of the adjustment of the detecting wavelength to 436 nm. The HPLC method was applicable to monitoring the fermentation and purification processes.
Authors:
T Ogasawara; S Goto; S Mori; T Oki
Related Documents :
9653957 - Stability-indicating analysis of isoxazolyl penicillins using dual wavelength high-perf...
9106067 - Determination of acyclovir and its metabolite 9-carboxymethoxymethylguanine in serum an...
10766367 - Validation of a method for the determination of zolpidem in human plasma using lc with ...
24163717 - Analyzing lc-ms/ms data by spectral count and ion abundance: two case studies.
23953437 - Ultrasound assisted dialysis of semi-permeable membrane devices for the simultaneous an...
7733427 - Folic acid fortification of grain: an economic analysis.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of antibiotics     Volume:  34     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-09-25     Completed Date:  1981-09-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  47-51     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aclarubicin
Chromatography, High Pressure Liquid / methods
Fermentation
Naphthacenes / analysis,  biosynthesis,  isolation & purification
Streptomyces / metabolism
Chemical
Reg. No./Substance:
0/Naphthacenes; 57576-44-0/Aclarubicin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Absorption, distribution and excretion of cefmenoxime (SCE-1365), a novel broad-spectrum cephalospor...
Next Document:  High performance liquid chromatographic determination of aclacinomycin a and its related compounds. ...