Document Detail

High-performance liquid chromatographic determination of naturally occurring folates during tempe preparation.
MedLine Citation:
PMID:  15612749     Owner:  NLM     Status:  MEDLINE    
A trienzyme treatment (protease, alpha-amylase, and human plasma conjugase), followed by purification using SPE with SAX cartridges and reversed-phase HPLC with UV-PDA detection, was performed for determination of the distribution of various folate forms and content at various stages of tempe preparation. The major folate form in soybean identified was 5-formyl tetrahydrofolate (5-CHO-H4folate), followed by 10-formyl tetrahydrofolate (10-CHO-PGA), and 5-methyl tetrahydrofolate (5-CH3-H4folate), whereas folic acid was not detected and tetrahydrofolic acid (H4folate) was not detectable. The most predominant form in tempe was also 5-CHO-H4folate, followed by 10-CHO-PGA, whereas the quantities of 5-CH3-H4folate and folic acid were negligible. Quantities and retention of folate significantly decreased during the first boiling, dehulling, soaking, and second boiling procedures, yielding folate retention of 32%. A remarkable increase in folate content was found after fermentation, 5.2-fold higher than that of the boiled soybean. This may be due to de novo formation of folate by Rhizopus oligosporus, the principal mold in tempe fermentation. HPLC results were approximately 38-55% lower than the values obtained from the microbiological assay using Lactobacillus casei.
Erliana Ginting; Jayashree Arcot
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  52     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-22     Completed Date:  2005-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7752-8     Citation Subset:  IM    
Indonesian Legume and Tuber Crops Research Institute (ILETRI), Malang, Indonesia.
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MeSH Terms
Chromatography, High Pressure Liquid*
Folic Acid / analysis*
Food Handling / methods*
Plant Proteins / chemistry*
Polysaccharides / chemistry*
Quality Control
Soy Foods
Reg. No./Substance:
0/Plant Proteins; 0/Polysaccharides; 0/okara, Glycine max; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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