Document Detail


High-performance liquid chromatographic assay for the simultaneous determination of ethyl clofibrate and clofibric acid in plasma. Evaluation of plasma stability of ethyl clofibrate polylactic nanocapsules in human and rat plasmas.
MedLine Citation:
PMID:  7866548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid, sensitive, and selective HPLC assay was developed for the simultaneous determination of ethyl clofibrate and its major metabolite, clofibric acid, in plasma of humans and rats. The assay involves extraction of the compounds into chloroform-isoamyl alcohol (99:1, v/v) from plasma acidified with sulfuric acid. For human plasma, the overall recoveries of ethyl clofibrate and clofibric acid were 63 and 90%, respectively. The limits of detection of the assay for ethyl clofibrate and clofibric acid in human plasma were 1.1 and 1.5 micrograms/ml, respectively. Limits of quantitation of the assay for ethyl clofibrate and clofibric acid in human plasma were 3.6 and 4.9 micrograms/ml, respectively. The HPLC assay was used to monitor the plasma concentration-time profiles of ethyl clofibrate released from polylactic nanocapsules both in man and rat. The simultaneous determination of ethyl clofibrate and clofibric acid provided evidence that these colloidal systems are stable in human plasma whereas they are lysed in rat plasma.
Authors:
J Barra; A M Taburet; A Jardel; H Fessi; F Puisieux
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  661     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-03-28     Completed Date:  1995-03-28     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  178-82     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Technology, Université de Paris-Sud, Châtenay-Malabry, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Capsules
Chromatography, High Pressure Liquid / methods*
Clofibrate / blood*
Clofibric Acid / blood*
Humans
Rats
Rats, Wistar
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Capsules; 637-07-0/Clofibrate; 882-09-7/Clofibric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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