| High-performance liquid chromatographic assay with fluorescence detection for the evaluation of inhibitors against fatty acid amide hydrolase. | |
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MedLine Citation:
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PMID: 19484225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fluorescent assay for the evaluation of inhibitors of fatty acid amide hydrolase (FAAH) is described. Microsomes from rat brain served as enzyme source. N-(2-Hydroxyethyl)-4-pyren-1-ylbutanamide was designed and synthesized as novel fluorogenic substrate. For substrate solubilization, Triton X-100 was employed. The FAAH activity was determined directly without further sample clean-up by measuring the amount of 4-pyren-1-ylbutanoic acid released by the enzyme with reversed-phase HPLC and fluorescence detection. The known FAAH inhibitors URB597, phenyl hexanoyl oxazolopyridine (PHOP) and [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamic acid phenyl ester were used to validate the test assay. |
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Authors:
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Laura Forster; Alwine Schulze Elfringhoff; Matthias Lehr |
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Publication Detail:
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Type: Journal Article; Validation Studies Date: 2009-05-30 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 394 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-24 Completed Date: 2009-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1679-85 Citation Subset: IM |
Affiliation:
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Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Hittorfstrasse 58-62, 48149 Münster, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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antagonists & inhibitors*,
metabolism Animals Brain / drug effects, enzymology Chromatography, High Pressure Liquid / methods* Enzyme Inhibitors / chemistry, pharmacology* Female Fluorescent Dyes / analysis*, chemical synthesis, chemistry, metabolism Molecular Structure Rats Rats, Sprague-Dawley Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Fluorescent Dyes; EC 3.5.-/Amidohydrolases; EC 3.5.1.-/fatty-acid amide hydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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