| High-performance liquid chromatographic assay for minocycline in human plasma and parotid saliva. | |
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MedLine Citation:
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PMID: 10718653 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive and specific high-performance liquid chromatographic (HPLC) method with UV detection was developed for the determination of minocycline in human plasma and parotid saliva samples. Samples were extracted using an Oasis HLB cartridge and were injected into a C8 Nucleosil column. The HPLC eluent contained acetonitrile-methanol-distilled water-0.1% trifluoroacetic acid (25:2:72.9:0.1, v/v). Demeclocycline was used as internal standard. The assay showed linearity in the tested range of 0.1-25 microg/ml. The limit of quantitation was 100 ng/ml. Recovery from plasma or parotid saliva averaged 95%. Precision expressed as %CV was in the range 0.2-17% (limit of quantitation). Accuracy ranged from 93 to 111%. In the two matrices studied at 20 and 4 degrees C, rapid degradation of the drug occurred. Frozen at -30 degrees C, this drug was stable for at least 2 months, the percent recovery averaged 90%. The method's ability to quantify minocycline with precision, accuracy and sensitivity makes it useful in pharmacokinetic studies. |
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Authors:
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V Orti; M Audran; P Gibert; G Bougard; F Bressolle |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 738 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-05-05 Completed Date: 2000-05-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 357-65 Citation Subset: IM |
Affiliation:
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Laboratoire de Pharmacocinétique, Faculté de Pharmacie, Montpellier, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid
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methods* Humans Minocycline / analysis*, blood Parotid Gland / secretion* Reference Standards Reproducibility of Results Saliva / chemistry* Sensitivity and Specificity Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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10118-90-8/Minocycline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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