| High-performance liquid chromatographic analysis of the new antitumour drug N-benzoylstaurosporine (CGP 41 251) and four potential metabolites in micro-volumes of plasma. | |
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MedLine Citation:
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PMID: 8833979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high-performance liquid chromatographic (HPLC) assay is described for the determination of the new antitumour drug N-benzoylstaurosporine (CGP 41 251; I) and four of its potential metabolites in micro-volumes (100 microliters) of plasma. After addition of an internal standard, the compounds were isolated from plasma by liquid-liquid extraction with diisopropyl ether. Chromatography was carried out using a 5 microns LiChrospher C-18 end-capped column (125 x 4.0 mm i.d.) and binary gradient elution with acetonitrile and a triethylamine-containing phosphate buffer (pH 3.6) as solvents. Fluorimetric detection was performed with excitation and emission wavelengths set at 286 and 386 nm, respectively. The absolute recovery was more than 98% for all of the investigated compounds. The limit of detection (LOD) for I and three metabolites was 0.1 ng ml-1 and the lower limit of quantitation (LLQ) was 0.2 ng ml-1 in 100 microliters of plasma. The LOD and LLQ for the fourth metabolite was 0.25 and 0.5 ng ml-1, respectively. The between-day and within-day precisions were always < 15% for all the analytes. A limited pharmacokinetic study in mice treated and with I demonstrated that the method is appropriate for this purpose. |
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Authors:
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R van Gijn; E Havik; E Boven; J B Vermorken; W W ten Bokkel Huinink; O van Tellingen; J H Beijnen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 14 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1997-01-02 Completed Date: 1997-01-02 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 165-74 Citation Subset: IM |
Affiliation:
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Department of Pharmacy, Slotervaart Hospital/Netherlands Cancer Institute, Amsterdam. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / blood*, pharmacokinetics Biotransformation Calibration Chromatography, High Pressure Liquid Male Mice Mice, Inbred Strains Quality Control Rats Reference Standards Solutions Staurosporine / analogs & derivatives*, blood, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Solutions; 120685-11-2/4'-N-benzoylstaurosporine; 62996-74-1/Staurosporine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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