Document Detail


High-performance liquid chromatographic analysis of the new antitumour drug N-benzoylstaurosporine (CGP 41 251) and four potential metabolites in micro-volumes of plasma.
MedLine Citation:
PMID:  8833979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high-performance liquid chromatographic (HPLC) assay is described for the determination of the new antitumour drug N-benzoylstaurosporine (CGP 41 251; I) and four of its potential metabolites in micro-volumes (100 microliters) of plasma. After addition of an internal standard, the compounds were isolated from plasma by liquid-liquid extraction with diisopropyl ether. Chromatography was carried out using a 5 microns LiChrospher C-18 end-capped column (125 x 4.0 mm i.d.) and binary gradient elution with acetonitrile and a triethylamine-containing phosphate buffer (pH 3.6) as solvents. Fluorimetric detection was performed with excitation and emission wavelengths set at 286 and 386 nm, respectively. The absolute recovery was more than 98% for all of the investigated compounds. The limit of detection (LOD) for I and three metabolites was 0.1 ng ml-1 and the lower limit of quantitation (LLQ) was 0.2 ng ml-1 in 100 microliters of plasma. The LOD and LLQ for the fourth metabolite was 0.25 and 0.5 ng ml-1, respectively. The between-day and within-day precisions were always < 15% for all the analytes. A limited pharmacokinetic study in mice treated and with I demonstrated that the method is appropriate for this purpose.
Authors:
R van Gijn; E Havik; E Boven; J B Vermorken; W W ten Bokkel Huinink; O van Tellingen; J H Beijnen
Related Documents :
436249 - Improved liquid-chromatographic determination of propranolol in plasma, with fluorescen...
12450519 - Assay methodology for the quantitation of unbound ertapenem, a new carbapenem antibioti...
9869379 - Determination of pnu 157706, a new dual inhibitor of 5alpha-reductase, in rat plasma by...
8207939 - Determination of albendazole metabolites in plasma by hplc.
22740009 - Molecular approaches to allergen standardization.
7428179 - A direct analysis of methotrexate, dichloromethotrexate and their 7-hydroxy metabolites...
21218069 - Development and validation of hplc method for the determination of pregabalin in capsules.
9106059 - Application of capillary zone electrophoresis in cephalosporin analysis.
8228979 - Purification, composition, charge, and molecular weight of the femo cofactor from azoto...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  14     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1997-01-02     Completed Date:  1997-01-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  165-74     Citation Subset:  IM    
Affiliation:
Department of Pharmacy, Slotervaart Hospital/Netherlands Cancer Institute, Amsterdam.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / blood*,  pharmacokinetics
Biotransformation
Calibration
Chromatography, High Pressure Liquid
Male
Mice
Mice, Inbred Strains
Quality Control
Rats
Reference Standards
Solutions
Staurosporine / analogs & derivatives*,  blood,  pharmacokinetics
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Solutions; 120685-11-2/4'-N-benzoylstaurosporine; 62996-74-1/Staurosporine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Determination of the pharmaceutical peptide TP9201 by post-column reaction with copper(II) followed ...
Next Document:  Simultaneous determination of 5-aminosalicylic acid, acetyl-5-aminosalicylic acid and 2,5-dihydroxyb...