Document Detail


High performance liquid chromatographic analysis of catecholamines in growing and non-growing Tetrahymena pyriformis.
MedLine Citation:
PMID:  6789887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tetrahymena pyriformis, strains NT-1 and W, harvested in logarithmic (growing) and stationary (non-growing) phases, were found by high-performance liquid chromatography to contain considerable quantities of dopamine. In addition, small amounts of epinephrine and norepinephrine were detected. Logarithmic-phase strain NT-1 cells contained 249 +/- 44 pg dopamine/10(6) cells compared to 477 +/- 42 pg/10(6) cells for logarithmic-phase strain W cells for logarithmic-phase strain W cells. The dopamine content of stationary-phase cells was approximately half the value of the logarithmic-phase cells. There was a significant amount of dopamine in the growth medium from stationary-phase cultures and, to a lesser extent, logarithmic-phase cells.
Authors:
M E Goldman; R E Gundersen; C K Erickson; G A Thompson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  676     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1981 Aug 
Date Detail:
Created Date:  1981-10-25     Completed Date:  1981-10-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  221-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Catecholamines / metabolism*
Chromatography, High Pressure Liquid
Dopamine / metabolism
Epinephrine / metabolism
Norepinephrine / metabolism
Tetrahymena pyriformis / growth & development*,  metabolism
Grant Support
ID/Acronym/Agency:
1TE32CA09182/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Catecholamines; 51-41-2/Norepinephrine; 51-43-4/Epinephrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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