Document Detail

High-performance liquid chromatographic analysis of oxytetracycline in chinook salmon following administration of medicated feed.
MedLine Citation:
PMID:  1818082     Owner:  NLM     Status:  MEDLINE    
A high-performance liquid chromatographic assay was developed to detect oxytetracycline (OTC) in chinook salmon muscle tissue. A solid-phase extraction protocol was used to recover OTC and the internal standard, epitetracycline hydrochloride, from the salmon tissue samples. OTC was analyzed using a mobile phase of methanol-0.02 M phosphate buffer, pH 2.25 (60:190), an ultraviolet detection wavelength of 365 nm and 250 mm x 4.6 mm I.D. Ultrasphere ODS column. A linear calibration curve (r2 = 0.999) of OTC in salmon muscle tissue from 0.05 to 3.0 ppm was obtained. Using a signal-to-noise ratio of 5:1, the OTC detection limit was 0.5 ppm in salmon muscle tissue. OTC recovery (74.4%) and intra-assay variability (2.3%) were optimized for salmon muscle tissue. An in vivo feeding study was performed by administrating OTC-medicated feed for a period of 10 days, followed by a 42-day sampling period. The half-life for the elimination of OTC in chinook salmon muscle tissue was found to be 5.4 days.
R G Aoyama; K M McErlane; H Erber; D D Kitts; H M Burt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  588     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-07-13     Completed Date:  1992-07-13     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  181-6     Citation Subset:  IM    
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MeSH Terms
Animal Feed*
Chromatography, High Pressure Liquid / methods*,  statistics & numerical data
Muscles / chemistry*
Oxytetracycline / administration & dosage,  analysis*,  pharmacokinetics
Reg. No./Substance:

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