| High-performance liquid chromatographic analysis of amino acids in ackee fruit with emphasis on the toxic amino acid hypoglycin A. | |
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MedLine Citation:
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PMID: 12387335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High-performance liquid chromatography is used to determine the amino acid content of ripe and unripe ackee fruit. Specific emphasis is placed on the level of the toxic amino acid hypoglycin A (hyp-A) in the unripe and ripe ackee fruit and seed. Unripe samples are found to contain significantly higher quantities (P < 0.05) of hyp-A when compared with ripe samples. Uncooked unripe fruit is found to contain 124.4 +/- 6.7 mg/100 g fresh weight and uncooked ripe fruit 6.4 +/- 1.1 mg/100 g fresh weight. The seed of the uncooked unripe fruit is found to contain 142.8 +/- 8.8 mg/100 g fresh weight, and the seed of uncooked ripe fruit has 106.0 +/- 5.4 mg/100 g fresh weight. Boiling fruit in water for approximately 30 min is efficient in removing hyp-A from the edible arilli; however, low levels of 0.54 +/- 0.15 mg/200 mL are detected in the water that was used to cook the ripe fruit. The average %recovery of the amino acids was 80.34%. |
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Authors:
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K D Golden; O J Williams; Y Bailey-Shaw |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatographic science Volume: 40 ISSN: 0021-9665 ISO Abbreviation: J Chromatogr Sci Publication Date: 2002 Sep |
Date Detail:
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Created Date: 2002-10-21 Completed Date: 2002-11-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0173225 Medline TA: J Chromatogr Sci Country: United States |
Other Details:
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Languages: eng Pagination: 441-6 Citation Subset: IM |
Affiliation:
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Department of Basic Medical Sciences, University of the West Indies, Kingston. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Chromatography, High Pressure Liquid / methods* Cookery Hypoglycins / analysis* Plants, Edible / chemistry* Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Hypoglycins; 156-56-9/hypoglycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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