Document Detail

High osmotic pressure enables fine ultrastructural and cytochemical studies on Pneumocystis carinii. I. Epon embedding.
MedLine Citation:
PMID:  1495924     Owner:  NLM     Status:  MEDLINE    
High osmotic pressure was used to preserve the ultrastructure of rabbit-, SCID mouse-, and rat-derived Pneumocystis carinii organisms from osmotic stress during fixation. Organelles and cytosol were well preserved within the tonicity range of 850-1,300 mosmol. Under these experimental conditions, we determined that the endoplasmic reticulum was well developed in all parasite stages and could observe the Golgi complex, autophagic vacuoles, dense bodies, type II endoplasmic saccules, and the recently described outer surface membrane, which was found in all parasite stages. The biological implications of these findings are discussed.
F Palluault; C Slomianny; B Soulez; E Dei-Cas; D Camus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parasitology research     Volume:  78     ISSN:  0932-0113     ISO Abbreviation:  Parasitol. Res.     Publication Date:  1992  
Date Detail:
Created Date:  1992-09-04     Completed Date:  1992-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  437-44     Citation Subset:  IM    
I.N.S.E.R.M. (U. 42), Unité de Biologie et Biochimie Parasitaires et Fongiques, domaine du C.E.R.T.I.A., Villeneuve d'Ascq, France.
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MeSH Terms
Cell Nucleus / ultrastructure
Cytosol / ultrastructure
Endoplasmic Reticulum / ultrastructure
Epoxy Resins*
Golgi Apparatus / ultrastructure
Microscopy, Electron
Osmolar Concentration
Osmotic Pressure
Plastic Embedding / methods*
Pneumocystis / ultrastructure*
Vacuoles / ultrastructure
Reg. No./Substance:
0/Epoxy Resins; 67076-53-3/EPON

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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