Document Detail


High occurrence of Helicobacter pylori in raw goat, sheep and cow milk inferred by glmM gene: a risk of food-borne infection?
MedLine Citation:
PMID:  18372071     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Helicobacter pylori is an organism widespread in humans and sometimes responsible for serious illnesses, such as gastric and duodenal ulcers, MALToma and even gastric cancer. It has been hypothesized that the infection route by H. pylori involves multiple pathways including food-borne transmission, as the microorganism has been detected from foods such as sheep and cow milk. This work reports the results of a survey conducted in order to investigate the presence of H. pylori in raw goat, sheep and cow milk produced in Southern Italy, employing a Nested Polymerase Chain Reaction (Nested-PCR) assay for the detection of the phosphoglucosamine mutase gene (glmM), as screening method followed by conventional bacteriological isolation. Out of the 400 raw milk samples examined, 139 (34.7%) resulted positive for the presence of glmM gene, but no strains were isolated. In this work H. pylori DNA has been firstly detected from 41 (25.6%) raw goat milk samples. The results deserve further investigations on the contamination source/s of the milk samples and on the major impact that it may have on consumers.
Authors:
N C Quaglia; A Dambrosio; G Normanno; A Parisi; R Patrono; G Ranieri; A Rella; G V Celano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-21
Journal Detail:
Title:  International journal of food microbiology     Volume:  124     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-13     Completed Date:  2008-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  43-7     Citation Subset:  IM    
Affiliation:
Department of Health and Animal Welfare, Faculty of Veterinary Medicine, Strada prov. Per Casamassima Km 3, 70010 Valenzano (Bari) Italy. n.quaglia@libero.it <n.quaglia@libero.it>
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Colony Count, Microbial
Consumer Product Safety*
DNA, Bacterial / chemistry,  genetics
Food Contamination / analysis*
Food Microbiology
Goats
Helicobacter Infections / transmission
Helicobacter pylori / enzymology,  genetics,  isolation & purification*
Humans
Milk / microbiology*
Phosphoglucomutase / genetics*
Polymerase Chain Reaction / methods
Sheep
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Bacterial; EC 5.4.2.-/phosphoglucosamine mutase; EC 5.4.2.2/Phosphoglucomutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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