Document Detail


High molecular mass phosphoproteins in the rat liver nuclear matrix identification of a prominent 110,000 Dalton species.
MedLine Citation:
PMID:  6224486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Purified rat liver nuclei were labelled in vitro in the presence of (32P) ATP and submitted to sequential extraction with DNAse, 0.4 or 2.0 M NaCl and Triton X-100. The residual or matrix structures contained 8-10 phosphoproteins between 76 and 260 kd including a triplet of major bands with 110, 117 and 128 kd. The 110 kd species was purified by chromatography on oligo(dT)-cellulose. It was shown to be identical with the 110 kd phosphoprotein of rat liver or Morris hepatoma free polyribosomes using the technique of limited digestion with S. aureus protease V 8.
Authors:
A Schweiger; G Kostka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  114     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-09-09     Completed Date:  1983-09-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  183-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / analysis*
Chromatography, Affinity
Deoxyribonuclease I
Electrophoresis, Polyacrylamide Gel
Endodeoxyribonucleases
Liver / analysis*
Molecular Weight
Phosphoproteins / isolation & purification*
Rats
Chemical
Reg. No./Substance:
0/Phosphoproteins; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.1/Deoxyribonuclease I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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