| High mobility of proteins in the mammalian cell nucleus. | |
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MedLine Citation:
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PMID: 10766243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mammalian cell nucleus contains numerous sub-compartments, which have been implicated in essential processes such as transcription and splicing. The mechanisms by which nuclear compartments are formed and maintained are unclear. More fundamentally, it is not known how proteins move within the cell nucleus. We have measured the kinetic properties of proteins in the nucleus of living cells using photobleaching techniques. Here we show that proteins involved in diverse nuclear processes move rapidly throughout the entire nucleus. Protein movement is independent of energy, which indicates that proteins may use a passive mechanism of movement. Proteins rapidly associate and dissociate with nuclear compartments. Using kinetic modelling, we determined residence times and steady-state fluxes of molecules in two main nuclear compartments. These data show that many nuclear proteins roam the cell nucleus in vivo and that nuclear compartments are the reflection of the steady-state association/dissociation of its 'residents' with the nucleoplasmic space. Our observations have conceptual implications for understanding nuclear architecture and how nuclear processes are organized in vivo. |
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Authors:
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R D Phair; T Misteli |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature Volume: 404 ISSN: 0028-0836 ISO Abbreviation: Nature Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-04-27 Completed Date: 2000-04-27 Revised Date: 2011-09-27 |
Medline Journal Info:
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Nlm Unique ID: 0410462 Medline TA: Nature Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 604-9 Citation Subset: IM |
Affiliation:
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BioInformatics Services, Rockville, Maryland 20854, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport Cell Line Cell Nucleus / metabolism* Chromosomal Proteins, Non-Histone / metabolism Fluorescence Hela Cells High Mobility Group Proteins / metabolism Humans Kinetics Nuclear Proteins / metabolism* Recombinant Fusion Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chromosomal Proteins, Non-Histone; 0/High Mobility Group Proteins; 0/Nuclear Proteins; 0/Recombinant Fusion Proteins; 0/fibrillarin; 170974-22-8/serine-arginine-rich splicing proteins |
| Comments/Corrections | |
Comment In:
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Nat Rev Mol Cell Biol. 2010 Oct;11(10):678-9
[PMID:
20861872
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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