Document Detail

High-mobility group proteins 14 and 17 maintain the timing of early embryonic development in the mouse.
MedLine Citation:
PMID:  11133167     Owner:  NLM     Status:  MEDLINE    
The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. We report that both mRNA species and both proteins are present throughout oogenesis and preimplantation development of the mouse. When antisense oligonucleotides targeting each mRNA species are injected into one-cell embryos, the proteins become depleted at the two- and four-cell stages and reaccumulate at the eight-cell stage. One-cell embryos injected with antisense oligonucleotides targeting both HMG-14 and HMG-17 cleave to the two-cell stage. Subsequent cleavages, however, are delayed compared with control-injected embryos. Nevertheless, these embryos ultimately reach the blastocyst stage. Similarly, injection into the nuclei of two-cell embryos of a peptide corresponding to the common nucleosome-binding domain of HMG-14 and HMG-17 delays progression to the four-cell stage. Furthermore, both RNA and protein synthesis is transiently reduced in antisense-injected embryos compared with injected controls. These results identify HMG-14 and HMG-17 as constitutive components of mouse oocyte and embryonic chromatin and establish a link between the structure of embryonic chromatin and the normal progression of embryonic development.
O A Mohamed; M Bustin; H J Clarke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  229     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-02-08     Completed Date:  2001-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-49     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Obstetrics & Gynecology, McGill University, Montreal, Quebec, H3A 1A1, Canada.
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MeSH Terms
Binding Sites
Blastocyst / drug effects,  physiology*
Cell Cycle / physiology
Chromatin / metabolism*
High Mobility Group Proteins / metabolism*
Oligonucleotides, Antisense / pharmacology
Oocytes / physiology
Oogenesis / drug effects,  physiology*
Peptide Fragments / pharmacology
Protein Binding
Protein Biosynthesis
Time Factors
Transcription, Genetic
Reg. No./Substance:
0/Chromatin; 0/High Mobility Group Proteins; 0/Oligonucleotides, Antisense; 0/Peptide Fragments

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