| High levels of proteolytic enzymes in the ascitic fluid and plasma of rats bearing the Yoshida AH-130 hepatoma. | |
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MedLine Citation:
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PMID: 8621267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasma and ascitic fluid of rats bearing the Yoshida ascites hepatoma AH-130 were shown to contain high levels of proteolytic enzymes belonging to different classes active at neutral and acidic pH. Relative to those measured in control rat plasma, in tumor-bearing animals, the activity levels of lysosomal cathepsins B and L, in their latent, acidic-activatable form, were approximately 5-fold higher in plasma and 9-fold higher in ascitic fluid, and cathepsin D activity was about 5-fold higher in both plasma and ascitic fluid. Plasma and ascitic fluid of tumor-bearing rats also contained novel neutral and acidic gelatinolytic activities. The latter, as revealed by zymographic analysis conducted at pH 6.0, in the presence of dithiothreitol and in the absence of divalent metal ions, was sensitive to iodoacetamide inhibition but not to EDTA, showed a molecular mass of approximately 90 kD on SDS-PAGE, and was lost upon limited proteolysis with pepsin. Therefore, this enzyme is not identifiable as cathepsin B or L or their related latent forms and may represent a novel, so far undescribed, gelatinase. Its presence exclusively in the body fluids of AH-130-bearing rats suggests its possible use as a tumor marker. |
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Authors:
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C Isidoro; M Démoz; D De Stefanis; F M Baccino; G Bonelli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Invasion & metastasis Volume: 15 ISSN: 0251-1789 ISO Abbreviation: Invasion Metastasis Publication Date: 1995 |
Date Detail:
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Created Date: 1996-06-18 Completed Date: 1996-06-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8202435 Medline TA: Invasion Metastasis Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 116-24 Citation Subset: IM |
Affiliation:
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Dipartimento di Medicina et Oncologia Sperimentale, Università di Torino (I), Italia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascitic Fluid / enzymology*, metabolism Cathepsins / blood, metabolism Cell Count Electrophoresis, Polyacrylamide Gel Gelatinases / blood, metabolism Hydrogen-Ion Concentration Liver Neoplasms, Experimental / enzymology*, metabolism, pathology Lysosomes / metabolism Male Peptide Hydrolases / blood, metabolism* Rats Rats, Wistar Tumor Markers, Biological / blood, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Tumor Markers, Biological; EC 3.4.-/Cathepsins; EC 3.4.-/Peptide Hydrolases; EC 3.4.24.-/Gelatinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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