Document Detail

High level production of adrenic acid in Physcomitrella patens using the algae Pavlova sp. Delta(5)-elongase gene.
MedLine Citation:
PMID:  20106658     Owner:  NLM     Status:  MEDLINE    
Adrenic acid (ADA), an omega-6 polyunsaturated fatty acid (PUFA), has attracted much interest due to its pharmaceutical potential. Exploiting the wealth of information currently available on in planta oil biosynthesis, and coupling this information with the tool of genetic engineering, it is now feasible to deliberately alter fatty acid biosynthetic pathways to generate unique oils in commodity crops. In this study, a Delta(5)-elongase gene from the algae Pavlova sp. related to the biosynthesis of C(22) PUFAs was targeted to enable production of ADA in the moss Physcomitrella patens. Heterologous expression of this gene was under the control of a tandemly duplicate 35S promoter. It was established that ADA (0.42mg/l) was synthesized in P. patens from endogenous arachidonic acid (ARA) via the expressed Pavlova sp. Delta(5)-elongase in the moss. In an attempt to maximize ADA production, medium optimization was effected by the response surface methodology (RSM), resulting in a significant elevation of ADA (4.51mg/l) production under optimum conditions. To the best of our knowledge, this is the first study describing the expression of a PUFA synthesizing enzyme in non-seed lower plant without supplying the exogenous fatty acid.
Songsri Kaewsuwan; Nantavan Bunyapraphatsara; David J Cove; Ralph S Quatrano; Pichit Chodok
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-27
Journal Detail:
Title:  Bioresource technology     Volume:  101     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-02-23     Completed Date:  2010-06-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  4081-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Marine Natural Products Research Unit, Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkla 90112, Thailand.
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MeSH Terms
Acetyltransferases / genetics*
Algae / enzymology,  genetics*
Arachidonic Acid / biosynthesis
Blotting, Southern
Bryophyta / metabolism*
Erucic Acids / metabolism*
Fatty Acids, Unsaturated / analysis
Gas Chromatography-Mass Spectrometry
Reg. No./Substance:
0/Erucic Acids; 0/Fatty Acids, Unsaturated; 2091-25-0/adrenic acid; 506-32-1/Arachidonic Acid; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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