Document Detail


High level of messenger RNA for BRMS1 in primary breast carcinomas is associated with poor prognosis.
MedLine Citation:
PMID:  17163420     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BRMS1 is regarded as a metastasis suppressor gene for its ability to reduce metastatic potential of human and murine breast cancer cells as well as human melanoma cells. However, BRMS1 association to human tumor progression is not clearly understood. In the present study we analyzed BRMS1 mRNA expression in tumor progression and its potential prognostic value for breast carcinoma. BRMS1 mRNA expression level was quantified by real-time PCR in 47 tumoral, in 14 peritumoral and in 15 metastatic microdissected cellular populations from 47 breast cancer patients with 10-year follow up. We found BRMS1 expression to be higher in carcinoma cells than in matching normal epithelial cell populations in 10 out of 14 cases (p = 0.0005), while lymph-nodal carcinoma cells showed lower BRMS1 expression in 9 out of 15 cases (p = 0.001). Using both in vivo (human mammary breast carcinomas) and in vitro systems (breast cancer cell lines) we were able to demonstrate that BRMS1 overexpression was not a bias effect induced by cell proliferation rate. BRMS1 expression levels did not correlate with standard breast cancer prognostic factors but BRMS1 higher expression was associated with patient shorter disease-free and overall survival. Our findings are apparently inconsistent with the concept of BRMS1 as a metastasis suppressor gene. One possible explanation is that epithelial cells increase their BRMS1 expression as a compensatory response to tumor formation or metastasis progression, which is elevated in proportion to tumor aggressiveness, whereas those cells of the primary tumor that cannot upregulate BRMS1 escape to form metastasis.
Authors:
Grazia Lombardi; Claudio Di Cristofano; Alessandra Capodanno; Maria Carla Iorio; Paolo Aretini; Patrizia Isola; Mariella Tancredi; Paola Collecchi; Antonio Giuseppe Naccarato; Romana Prosperi Porta; Generoso Bevilacqua; Maria Adelaide Caligo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  120     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-05     Completed Date:  2007-04-24     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1169-78     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley-Liss, Inc.
Affiliation:
Division of Surgical, Molecular and Ultrastructural Pathology, Department of Oncology, University of Pisa and Pisa University Hospital, Via Roma 57, 56126 Pisa, Italy.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / diagnosis*,  pathology
Carcinoma / diagnosis*,  secondary
Epithelium / pathology
Female
Gene Expression
Genes, Tumor Suppressor*
Humans
Neoplasm Proteins / genetics*
RNA, Messenger / analysis*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/BRMS1 protein, human; 0/Neoplasm Proteins; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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