Document Detail

High-level expression of human c-Src can cause a spherical morphology without loss of anchorage-dependent growth of NIH 3T3 cells.
MedLine Citation:
PMID:  9271228     Owner:  NLM     Status:  MEDLINE    
To investigate whether overexpression of human c-Src leads to cell rounding in anchorage-dependent NIH 3T3 fibroblasts, we have established c-Src-inducible cell lines using a lac repressor-operator system. RN1 cells, which expressed c-Src at a high level after induction, exhibited a spherical morphology and ceased to grow in monolayer culture. RN1 cells, however, exhibited neither focus-forming ability nor anchorage-independent growth potential with or without induction. Induced RN1 c-Src was phosphorylated at Ser75, a previously reported spherical cell-associated site, and at Tyr419. These data demonstrated for the first time that highly elevated human c-Src tyrosine kinase activity can cause NIH 3T3 cells to have a spherical morphology without loss of anchorage-dependent growth. The inducible cell line should be useful to study the mechanism for cell rounding by c-Src.
G Kato; S Maeda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  411     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-09-23     Completed Date:  1997-09-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  317-21     Citation Subset:  IM    
Department of Biochemistry, Yamanashi Medical University, Nakakoma, Japan.
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MeSH Terms
3T3 Cells
Bacterial Proteins / genetics
Cell Adhesion / genetics
Cell Division*
Cell Size*
Electrophoresis, Polyacrylamide Gel
Escherichia coli Proteins*
Gene Expression Regulation / genetics*
Genes, src*
Isopropyl Thiogalactoside / pharmacology
Lac Repressors
Peptide Mapping
Phosphopeptides / analysis,  chemistry
Phosphoserine / metabolism
Precipitin Tests
Proto-Oncogene Proteins pp60(c-src) / metabolism
Repressor Proteins / genetics
Transfection / genetics
Reg. No./Substance:
0/Bacterial Proteins; 0/Escherichia coli Proteins; 0/Lac Repressors; 0/Phosphopeptides; 0/Repressor Proteins; 17885-08-4/Phosphoserine; 367-93-1/Isopropyl Thiogalactoside; EC Proteins pp60(c-src)

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