Document Detail


High-level expression of fusion protein containing 10 tandem repeated GLP-1 analogs in yeast Pichia pastoris and its biological activity in a diabetic rat model.
MedLine Citation:
PMID:  17587676     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glucagon-like peptide-1 (GLP-1), an incretin secreted by intestinal L-cells, can effectively lower blood glucose levels in patients with diabetes. A fusion gene, consisting of 10 tandem repeated GLP-1 analog genes, was expressed at a high level in the yeast Pichia pastoris. SDS polyacrylamide gel electrophoresis (SDS-PAGE), and Western Blotting results showed that fusion protein migrated as a single protein band with a molecular weight of 36 kDa. A biological activity test showed that the GLP-1 analog could significantly lower the level of serum glucose when GLP-1 purified analog was injected into diabetic rats. A potential strategy for large-scale production of fusion protein containing the 10 GLP-1 analogs as discovered, and a single GLP-1 analog was obtained from fusion protein digested with trypsin. This should be inspired foreign expression of medicinal short peptides and be valuable in further research on GLP-1 analog drugs in the treatment of diabetes mellitus.
Authors:
Jianhua Hou; Ruixiang Yan; Liquan Yang; Zhiqiang Wu; Cuiyan Wang; Dongfeng Ding; Na Li; Chunxiao Ma; Minggang Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  71     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-25     Completed Date:  2007-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1462-9     Citation Subset:  IM    
Affiliation:
The Key Laboratory for Bioactive Materials of the Ministry of Education, Institute for Molecular Biology, College of Life Science, Nankai University, P. R. China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / drug effects
Cloning, Molecular / methods*
Diabetes Mellitus / drug therapy*
Disease Models, Animal
Glucagon-Like Peptide 1 / analogs & derivatives,  genetics*,  therapeutic use*
Pichia / genetics*
Rats
Recombinant Fusion Proteins / genetics,  therapeutic use
Tandem Repeat Sequences
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Recombinant Fusion Proteins; 89750-14-1/Glucagon-Like Peptide 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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